Evaluation of Repetitive Element Sequence-based PCR As a Molecular Typing Method for Clostridium Difficile

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 2003 Jun 7

PMID 12791864

Citations 11

Authors

Patrizia Spigaglia

Paola Mastrantonio

Affiliations

Soon will be listed here.

Abstract

Repetitive element sequence-based PCR (rep-PCR) is a typing method that enables the generation of DNA fingerprinting that discriminates bacterial strains. In this study, we evaluated the applicability of rep-PCR in typing Clostridium difficile clinical isolates. The results obtained by rep-PCR were compared with those obtained by pulsed-field gel electrophoresis (PFGE) and PCR ribotyping. A high correspondence between pattern differentiations produced by rep-PCR and PFGE was observed, whereas PCR ribotyping showed a lower level of discriminatory power.

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