A Comparison of Molecular Typing Methods Applied to Complex: Sequencing, Rep-PCR, and MLST

Overview

Journal Pathog Immun

Date 2017 Apr 22

PMID 28428984

Citations 5

Authors

Roberto A Viau

Lee M Kiedrowski

Barry N Kreiswirth

Mark Adams

Federico Perez

Dror Marchaim

Dubert M Guerrero

Keith S Kaye

Latania K Logan

Maria Virginia Villegas

Robert A Bonomo

Affiliations

Soon will be listed here.

Abstract

Molecular typing using repetitive sequenced-based PCR (rep-PCR) and sequencing were applied to a collection of diverse complex isolates. To determine the most practical method for reference laboratories, we analyzed 71 complex isolates from sporadic and outbreak occurrences originating from 4 geographic areas. While rep-PCR was more discriminating, sequencing provided a broader and a more objective geographical tracking method similar to multilocus sequence typing (MLST). In addition, we suggest that MLST may have higher discriminative power compared to sequencing, although rep-PCR remains the most discriminative method for local outbreak investigations. In addition, rep-PCR can be an effective and inexpensive method for local outbreak investigation.

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