Vitrification of Human Blastocysts Using the Cryoloop Method: Successful Clinical Application and Birth of Offspring

Overview

Journal J Assist Reprod Genet

Publisher Springer

Specialties Genetics
Reproductive Medicine

Date 2002 Aug 9

PMID 12166639

Citations 14

Authors

Michael L Reed

Michelle Lane

David K Gardner

Nancy L Jensen

Jim Thompson

Affiliations

Soon will be listed here.

Abstract

Purpose: The purpose was to investigate vitrification of human blastocysts using the cryoloop.

Methods: Thirteen couples elected to have blastocysts cryopreserved via vitrification (N = 54 blastocysts). Embryos were exposed to two vitrification solutions, pipetted onto the cryoloop then plunged into liquid nitrogen. On the day of embryo replacement, blastocysts were warmed by passage through three dilution media, and rinsed into culture medium.

Results: Four couples returned for cryopreserved embryo replacement. Fifteen blastocysts were warmed; all 15 (15/15; 100%) were recovered. Fifteen (15/15; 100%) blastocysts were deemed viable, and 13 were replaced. Two replacements did not result in pregnancy; one resulted in clinical pregnancy with a blighted ovum; one resulted in clinical pregnancy with the live, term birth of a healthy male infant. Two of 13 embryos implanted yielding an implantation rate of 15.4%.

Conclusions: These preliminary data suggest that blastocysts can be successfully vitrified using the cryoloop method.

Citing Articles

Comparison of the effect of two commercialized vitrification carriers on pregnancy outcomes in freeze-thaw cycles.

Zhu H, Li L, Zhang H, Jiang Y, Liu R, Xi Q J Int Med Res. 2023; 51(8):3000605231187948.

PMID: 37585737 PMC: 10416665. DOI: 10.1177/03000605231187948.

Comparative Assessment of Survival and Clinical Outcome Between Two Commercial Vitrification Kits with Different Warming Protocols After Blastocyst Culture: Potential Perspectives Toward Simplified Warming Procedures.

Gunst J, Vynck M, Hostens K, Standaert V, Roggeman S, van de Vijver A Reprod Sci. 2023; 30(11):3212-3221.

PMID: 37311945 PMC: 10643310. DOI: 10.1007/s43032-023-01281-1.

Artificial shrinkage of blastocoel using a laser pulse prior to vitrification improves clinical outcome.

Darwish E, Magdi Y J Assist Reprod Genet. 2016; 33(4):467-71.

PMID: 26843389 PMC: 4818639. DOI: 10.1007/s10815-016-0662-z.

Large-volume vitrification of human biopsied and non-biopsied blastocysts: a simple, robust technique for cryopreservation.

Reed M, Said A, Thompson D, Caperton C J Assist Reprod Genet. 2014; 32(2):207-14.

PMID: 25464896 PMC: 4354176. DOI: 10.1007/s10815-014-0395-9.

A randomized controlled trial comparing two vitrification methods versus slow-freezing for cryopreservation of human cleavage stage embryos.

Fasano G, Fontenelle N, Vannin A, Biramane J, Devreker F, Englert Y J Assist Reprod Genet. 2013; 31(2):241-7.

PMID: 24317854 PMC: 3933602. DOI: 10.1007/s10815-013-0145-4.

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