Optimization of a Dilution Method for Human Expanded Blastocysts Vitrified Using EM Grids After Artificial Shrinkage

Overview

Journal J Assist Reprod Genet

Publisher Springer

Specialties Genetics
Reproductive Medicine

Date 2006 Feb 10

PMID 16468090

Citations 4

Authors

So-Young Lee

Hyung-Jun Kim

Sung-Jin Park

Hye-Jin Yoon

San-Hyun Yoon

Kyung-Hoon Lee

Won-Don Lee

Jin-Ho Lim

Affiliations

Soon will be listed here.

Abstract

Purpose: To verify a more effective dilution method that can be applied to human expanded blastocysts that are vitrified after artificial shrinkage.

Methods: Surplus expanded blastocysts that remained after embryo transfer (ET) in in vitro fertilization (IVF) cycles, were cryopreserved. The blastocysts were vitrified on EM grids following artificial shrinkage. After thawing the blastocysts, cryoprotectants were diluted using either a 6- or 2-step method. We examined the survival rate and clinical outcome of blastocysts of 151 patients in our ET program after thawing.

Results: The survival rate of blastocysts that were thawed using a 2-step method (91.6%, 239/261) was comparable with that of the 6-step method (89%, 186/209). The clinical pregnancy rate (45.9%, 39/85) and implantation rate (24.1%, 53/220) were slightly higher in the 2-step method than in the 6-step method (40.9%, 27/66; 19.4%, 33/170).

Conclusions: Our data indicate that the 2-step dilution method could be a simpler and more effective protocol for human expanded blastocysts that are vitrified using EM-grid following artificial shrinkage.

Citing Articles

Effects of blastocyst artificial collapse prior to vitrification on hatching and survival rates and the expression of klf4 gene in mouse embryos.

Pooyanfar F, Foroutan T, Dashtizad M Vet Res Forum. 2018; 9(1):87-92.

PMID: 29719669 PMC: 5913566.

Short-term storage of tripronucleated human embryos.

Grau N, Aparicio B, Escrich L, Mercader A, Delgado A, Remohi J J Assist Reprod Genet. 2013; 30(8):1043-7.

PMID: 23820799 PMC: 3790122. DOI: 10.1007/s10815-013-0036-8.

Vitrification of mouse embryos using the thin plastic strip method.

Ryu E, Hur Y, Ann J, Maeng J, Park M, Park J Clin Exp Reprod Med. 2013; 39(4):153-60.

PMID: 23346525 PMC: 3548073. DOI: 10.5653/cerm.2012.39.4.153.

Application of two different synthetic sequential media for the human IVF-ET program: a prospective, randomized, and comparative study.

Yoon J, Yoon H, Juhn K, Ko J, Yoon S, Ko Y Clin Exp Reprod Med. 2012; 38(4):186-92.

PMID: 22384441 PMC: 3283082. DOI: 10.5653/cerm.2011.38.4.186.

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