Comparison of the Effect of Two Commercialized Vitrification Carriers on Pregnancy Outcomes in Freeze-thaw Cycles

Overview

Journal J Int Med Res

Publisher Sage Publications

Specialty General Medicine

Date 2023 Aug 16

PMID 37585737

Authors

Haibo Zhu

Linlin Li

Hongguo Zhang

Yuting Jiang

Ruizhi Liu

Qi Xi

Affiliations

Soon will be listed here.

Abstract

Objective: This study aimed to compare the effects of two brands of commercial vitrification carriers on pregnancy outcomes in freeze-thaw cycles.

Methods: We included 4871 patients who underwent a "freeze all" strategy using the commercial carriers J.Y. straw and OYASHIPS straw in the Reproductive Center of the First Hospital of Jilin University. The pregnancy outcomes of cleavage-stage embryos and blastocysts were studied separately. Detailed data and the safety of children born from mothers with the two types of carriers were also compared.

Results: Patients who used J.Y. straw had similar clinical pregnancy and live birth rates with one and two cleavage-stage embryo transplantation to those who used OYASHIPS straw. In patients who had blastocyst transplantation, the clinical pregnancy rate of one blastocyst transplanted in those who used OYASHIPS straw was significantly higher than that in those who used J.Y. straw (57.85% vs 47.09%). Among children born from mothers who used J.Y. straw, the congenital disability rate was significantly higher than that in those with OYASHIPS straw.

Conclusion: The OYASHIPS straw carrier is cheap and can achieve clinical pregnancy and live birth outcomes comparable to those of J.Y. straw. Therefore, OYASHIPS straw is a good alternative option.

References

AbdelHafez F, Xu J, Goldberg J, Desai N . Vitrification in open and closed carriers at different cell stages: assessment of embryo survival, development, DNA integrity and stability during vapor phase storage for transport. BMC Biotechnol. 2011; 11:29. PMC: 3079628. DOI: 10.1186/1472-6750-11-29. View

Niederberger C, Pellicer A, Cohen J, Gardner D, Palermo G, ONeill C . Forty years of IVF. Fertil Steril. 2018; 110(2):185-324.e5. DOI: 10.1016/j.fertnstert.2018.06.005. View

Chu D, Fu L, Zhou W, Li Y . Effects of different open cryo-carriers on embryo survival and clinical outcome in frozen embryo transfer cycle patients. Syst Biol Reprod Med. 2018; 64(2):138-145. DOI: 10.1080/19396368.2017.1419510. View

Ghasem S, Negar K . Effect of vitrification on number of inner cell mass in mouse blastocysts in conventional straw, closed pulled straw, open pulled straw and cryoloop carriers. J Pak Med Assoc. 2013; 63(4):486-9. View

Bielanski A, Vajta G . Risk of contamination of germplasm during cryopreservation and cryobanking in IVF units. Hum Reprod. 2009; 24(10):2457-67. DOI: 10.1093/humrep/dep117. View

Desai N, Goldberg J, Austin C, Falcone T . The new Rapid-i carrier is an effective system for human embryo vitrification at both the blastocyst and cleavage stage. Reprod Biol Endocrinol. 2013; 11:41. PMC: 3660183. DOI: 10.1186/1477-7827-11-41. View

Kuleshova L, Shaw J . A strategy for rapid cooling of mouse embryos within a double straw to eliminate the risk of contamination during storage in liquid nitrogen. Hum Reprod. 2000; 15(12):2604-9. DOI: 10.1093/humrep/15.12.2604. View

Zhang J, Wang Y, Liu H, Mao X, Chen Q, Fan Y . Effect of in vitro culture period on birth weight after vitrified-warmed transfer cycles: analysis of 4,201 singleton newborns. Fertil Steril. 2018; 111(1):97-104. DOI: 10.1016/j.fertnstert.2018.10.006. View

Reed M, Lane M, Gardner D, Jensen N, Thompson J . Vitrification of human blastocysts using the cryoloop method: successful clinical application and birth of offspring. J Assist Reprod Genet. 2002; 19(6):304-6. PMC: 3455218. DOI: 10.1023/a:1015789532736. View

10.

Liang J, Zhang Y, Yu Y, Sun W, Jing J, Liu R . Effect of chromosomal polymorphisms of different genders on fertilization rate of fresh IVF-ICSI embryo transfer cycles. Reprod Biomed Online. 2014; 29(4):436-44. DOI: 10.1016/j.rbmo.2014.06.011. View

11.

Ogura A . Development of reproductive engineering techniques at the RIKEN BioResource Center. Exp Anim. 2016; 66(1):1-16. PMC: 5300997. DOI: 10.1538/expanim.16-0074. View

12.

. Mature oocyte cryopreservation: a guideline. Fertil Steril. 2012; 99(1):37-43. DOI: 10.1016/j.fertnstert.2012.09.028. View

13.

Aizer A, Noach-Hirsh M, Dratviman-Storobinsky O, Haas J, Orvieto R . Does the number of embryos loaded on a single cryo-carrier affect post-vitrification survival rate?. Zygote. 2020; 29(1):87-91. DOI: 10.1017/S0967199420000453. View

14.

Castillo C, Harper J, Roberts S, ONeill H, Johnstone E, Brison D . The impact of selected embryo culture conditions on ART treatment cycle outcomes: a UK national study. Hum Reprod Open. 2020; 2020(1):hoz031. PMC: 7016773. DOI: 10.1093/hropen/hoz031. View

15.

El-Danasouri I, Selman H . Successful pregnancies and deliveries after a simple vitrification protocol for day 3 human embryos. Fertil Steril. 2001; 76(2):400-2. DOI: 10.1016/s0015-0282(01)01907-0. View

16.

Kader A, Choi A, Orief Y, Agarwal A . Factors affecting the outcome of human blastocyst vitrification. Reprod Biol Endocrinol. 2009; 7:99. PMC: 2757025. DOI: 10.1186/1477-7827-7-99. View

17.

Youm H, Choi J, Oh D, Rho Y . Closed versus open vitrification for human blastocyst cryopreservation: A meta-analysis. Cryobiology. 2017; 77:64-70. DOI: 10.1016/j.cryobiol.2017.05.006. View

18.

Zhu X, Huang C, Wu L, Deng Y, Lai X, Gu H . Perinatal Outcomes and Related Risk Factors of Single vs Twin Pregnancy Complicated by Gestational Diabetes Mellitus: Meta-Analysis. Comput Math Methods Med. 2022; 2022:3557890. PMC: 9273416. DOI: 10.1155/2022/3557890. View

19.

Vanderzwalmen P, Bertin G, Debauche C, Standaert V, Bollen N, van Roosendaal E . Vitrification of human blastocysts with the Hemi-Straw carrier: application of assisted hatching after thawing. Hum Reprod. 2003; 18(7):1504-11. DOI: 10.1093/humrep/deg298. View

20.

Zhou R, Zhang X, Huang L, Wang S, Li L, Dong M . The impact of different cycle regimens on birthweight of singletons in frozen-thawed embryo transfer cycles of ovulatory women. Fertil Steril. 2022; 117(3):573-582. DOI: 10.1016/j.fertnstert.2021.09.033. View