» Articles » PMID: 12058070

Human Chromosomes 9, 12, and 15 Contain the Nucleation Sites of Stress-induced Nuclear Bodies

Overview
Journal Mol Biol Cell
Date 2002 Jun 12
PMID 12058070
Citations 42
Authors
Affiliations
Soon will be listed here.
Abstract

We previously reported the identification of a novel nuclear compartment detectable in heat-shocked HeLa cells that we termed stress-induced Src-activated during mitosis nuclear body (SNB). This structure is the recruitment center for heat shock factor 1 and for a number of RNA processing factors, among a subset of Serine-Arginine splicing factors. In this article, we show that stress-induced SNBs are detectable in human but not in hamster cells. By means of hamster>human cell hybrids, we have identified three human chromosomes (9, 12, and 15) that are individually able to direct the formation of stress bodies in hamster cells. Similarly to stress-induced SNB, these bodies are sites of accumulation of hnRNP A1-interacting protein and heat shock factor 1, are usually associated to nucleoli, and consist of clusters of perichromatin granules. We show that the p13-q13 region of human chromosome 9 is sufficient to direct the formation of stress bodies in hamster>human cell hybrids. Fluorescence in situ hybridization experiments demonstrate that the pericentromeric heterochromatic q12 band of chromosome 9 and the centromeric regions of chromosomes 12 and 15 colocalize with stress-induced SNBs in human cells. Our data indicate that human chromosomes 9, 12, and 15 contain the nucleation sites of stress bodies in heat-shocked HeLa cells.

Citing Articles

Membraneless organelles in health and disease: exploring the molecular basis, physiological roles and pathological implications.

Li Y, Liu Y, Yu X, Xu Y, Pan X, Sun Y Signal Transduct Target Ther. 2024; 9(1):305.

PMID: 39551864 PMC: 11570651. DOI: 10.1038/s41392-024-02013-w.


Satellite RNAs: emerging players in subnuclear architecture and gene regulation.

Ninomiya K, Yamazaki T, Hirose T EMBO J. 2023; 42(18):e114331.

PMID: 37526230 PMC: 10505914. DOI: 10.15252/embj.2023114331.


A guide to membraneless organelles and their various roles in gene regulation.

Hirose T, Ninomiya K, Nakagawa S, Yamazaki T Nat Rev Mol Cell Biol. 2022; 24(4):288-304.

PMID: 36424481 DOI: 10.1038/s41580-022-00558-8.


Keeping up with the condensates: The retention, gain, and loss of nuclear membrane-less organelles.

Lacroix E, Audas T Front Mol Biosci. 2022; 9:998363.

PMID: 36203874 PMC: 9530788. DOI: 10.3389/fmolb.2022.998363.


Reversible phase separation of HSF1 is required for an acute transcriptional response during heat shock.

Zhang H, Shao S, Zeng Y, Wang X, Qin Y, Ren Q Nat Cell Biol. 2022; 24(3):340-352.

PMID: 35256776 DOI: 10.1038/s41556-022-00846-7.


References
1.
Sullivan G, Bridger J, Cuthbert A, Newbold R, Bickmore W, McStay B . Human acrocentric chromosomes with transcriptionally silent nucleolar organizer regions associate with nucleoli. EMBO J. 2001; 20(11):2867-74. PMC: 125486. DOI: 10.1093/emboj/20.11.2867. View

2.
Tani T, Derby R, Hiraoka Y, Spector D . Nucleolar accumulation of poly (A)+ RNA in heat-shocked yeast cells: implication of nucleolar involvement in mRNA transport. Mol Biol Cell. 1996; 7(1):173-92. PMC: 278621. DOI: 10.1091/mbc.7.1.173. View

3.
Fakan S . Perichromatin fibrils are in situ forms of nascent transcripts. Trends Cell Biol. 1994; 4(3):86-90. DOI: 10.1016/0962-8924(94)90180-5. View

4.
Antonacci R, Marzella R, Finelli P, Lonoce A, Forabosco A, Archidiacono N . A panel of subchromosomal painting libraries representing over 300 regions of the human genome. Cytogenet Cell Genet. 1995; 68(1-2):25-32. DOI: 10.1159/000133882. View

5.
Pombo A, Cuello P, Schul W, Yoon J, Roeder R, Cook P . Regional and temporal specialization in the nucleus: a transcriptionally-active nuclear domain rich in PTF, Oct1 and PIKA antigens associates with specific chromosomes early in the cell cycle. EMBO J. 1998; 17(6):1768-78. PMC: 1170524. DOI: 10.1093/emboj/17.6.1768. View