ORC Interacts with THSC/TREX-2 and Its Subunits Promote Nxf1 Association with MRNP and MRNA Export in Drosophila

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 2016 Mar 27

PMID 27016737

Citations 17

Authors

Daria Kopytova

Varvara Popova

Maria Kurshakova

Yulii Shidlovskii

Elena Nabirochkina

Alexander Brechalov

Georgii Georgiev

Sofia Georgieva

Affiliations

Soon will be listed here.

Abstract

The origin recognition complex (ORC) of eukaryotes associates with the replication origins and initiates the pre-replication complex assembly. In the literature, there are several reports of interaction of ORC with different RNAs. Here, we demonstrate for the first time a direct interaction of ORC with the THSC/TREX-2 mRNA nuclear export complex. The THSC/TREX-2 was purified from the Drosophila embryonic extract and found to bind with a fraction of the ORC. This interaction occurred via several subunits and was essential for Drosophila viability. Also, ORC was associated with mRNP, which was facilitated by TREX-2. ORC subunits interacted with the Nxf1 receptor mediating the bulk mRNA export. The knockdown of Orc5 led to a drop in the Nxf1 association with mRNP, while Orc3 knockdown increased the level of mRNP-bound Nxf1. The knockdown of Orc5, Orc3 and several other ORC subunits led to an accumulation of mRNA in the nucleus, suggesting that ORC participates in the regulation of the mRNP export.

Citing Articles

Point Mutations in the M Domain of PCID2 Impair Its Function in mRNA Export in Drosophila melanogaster.

Vdovina Y, Georgieva S, Kopytova D Dokl Biol Sci. 2024; 518(1):112-115.

PMID: 39128950 DOI: 10.1134/S0012496624600167.

TREX-2-ORC Complex of D. melanogaster Participates in Nuclear Export of Histone mRNA.

Kurshakova M, Yakusheva Y, Georgieva S Dokl Biochem Biophys. 2024; 514(1):11-15.

PMID: 38189888 PMC: 11021305. DOI: 10.1134/S160767292370059X.

The Human TREX-2 Complex Interacts with Subunits of the ORC Complex.

Kurshakova M, Georgieva S, Kopytova D Dokl Biochem Biophys. 2023; 513(1):346-349.

PMID: 38066323 PMC: 10810029. DOI: 10.1134/S1607672923700552.

Interaction of mRNA with the C-Terminal Domain of PCID2, a Subunit of the TREX-2 Complex, Is Required for Its Export from the Nucleus to the Cytoplasm in Drosophila melanogaster.

Vdovina Y, Georgieva S, Kopytova D Dokl Biochem Biophys. 2023; 513(1):328-331.

PMID: 38066318 PMC: 10810031. DOI: 10.1134/S1607672923700527.

DNA mimic foldamers affect chromatin composition and disturb cell cycle progression.

Kleene V, Corvaglia V, Chacin E, Forne I, Konrad D, Khosravani P Nucleic Acids Res. 2023; 51(18):9629-9642.

PMID: 37650653 PMC: 10570015. DOI: 10.1093/nar/gkad681.

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