Replication Initiation Patterns in the Beta-globin Loci of Totipotent and Differentiated Murine Cells: Evidence for Multiple Initiation Regions

Overview

Journal Mol Cell Biol

Specialty Cell Biology

Date 2002 Jan 5

PMID 11756541

Citations 28

Authors

Mirit I Aladjem

Luo Wei Rodewald

Chii Mai Lin

Sarah Bowman

Daniel M Cimbora

Linnea L Brody

Elliot M Epner

Mark Groudine

Geoffrey M Wahl

Affiliations

Soon will be listed here.

Abstract

The replication initiation pattern of the murine beta-globin locus was analyzed in totipotent embryonic stem cells and in differentiated cell lines. Initiation events in the murine beta-globin locus were detected in a region extending from the embryonic Ey gene to the adult betaminor gene, unlike the restricted initiation observed in the human locus. Totipotent and differentiated cells exhibited similar initiation patterns. Deletion of the region between the adult globin genes did not prevent initiation in the remainder of the locus, suggesting that the potential to initiate DNA replication was not contained exclusively within the primary sequence of the deleted region. In addition, a deletion encompassing the six identified 5' hypersensitive sites in the mouse locus control region had no effect on initiation from within the locus. As this deletion also did not affect the chromatin structure of the locus, we propose that the sequences determining both chromatin structure and replication initiation lie outside the hypersensitive sites removed by the deletion.

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