Multiple Sites of Replication Initiation in the Human Beta-globin Gene Locus

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 2001 Feb 13

PMID 11160905

Citations 23

Authors

S Kamath

M Leffak

Affiliations

Soon will be listed here.

Abstract

The cell cycle-dependent, ordered assembly of protein prereplicative complexes suggests that eukaryotic replication origins determine when genomic replication initiates. By comparison, the factors that determine where replication initiates relative to the sites of prereplicative complex formation are not known. In the human globin gene locus previous work showed that replication initiates at a single site 5' to the ss-globin gene when protein synthesis is inhibited by emetine. The present study has examined the pattern of initiation around the genetically defined ss-globin replicator in logarithmically growing HeLa cells, using two PCR-based nascent strand assays. In contrast to the pattern of initiation detected in emetine-treated cells, analysis of the short nascent strands at five positions spanning a 40 kb globin gene region shows that replication initiates at more than one site in non-drug-treated cells. Quantitation of nascent DNA chains confirmed that replication begins at several locations in this domain, including one near the initiation region (IR) identified in emetine-treated cells. However, the abundance of short nascent strands at another initiation site approximately 20 kb upstream is approximately 4-fold as great as that at the IR. The latter site abuts an early S phase replicating fragment previously defined at low resolution in logarithmically dividing cells.

Citing Articles

Dynamic regulation of histone H3K9 is linked to the switch between replication and transcription at the Dbf4 origin-promoter locus.

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A replicator-specific binding protein essential for site-specific initiation of DNA replication in mammalian cells.

Zhang Y, Huang L, Fu H, Smith O, Lin C, Utani K Nat Commun. 2016; 7:11748.

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Peaks cloaked in the mist: the landscape of mammalian replication origins.

Hyrien O J Cell Biol. 2015; 208(2):147-60.

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Chromatin structure and replication origins: determinants of chromosome replication and nuclear organization.

Smith O, Aladjem M J Mol Biol. 2014; 426(20):3330-41.

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Activation of a human chromosomal replication origin by protein tethering.

Chen X, Liu G, Leffak M Nucleic Acids Res. 2013; 41(13):6460-74.

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