Delayed Expulsion of the Nematode Trichinella Spiralis in Mice Lacking the Mucosal Mast Cell-specific Granule Chymase, Mouse Mast Cell Protease-1

Overview

Journal J Exp Med

Specialties Allergy & Immunology
General Medicine

Date 2000 Dec 20

PMID 11120781

Citations 112

Authors

P A Knight

S H Wright

C E Lawrence

Y Y Paterson

H R Miller

Affiliations

Soon will be listed here.

Abstract

Expulsion of gastrointestinal nematodes is associated with pronounced mucosal mast cell (MMC) hyperplasia, differentiation, and activation, accompanied by the systemic release of MMC granule chymases (chymotrypsin-like serine proteases). The beta-chymase mouse mast cell protease-1 (mMCP-1) is expressed predominantly by intraepithelial MMCs, and levels in the bloodstream and intestinal lumen are maximal at the time of worm expulsion in parasitized mice. To address the in vivo functions of MMC-specific beta-chymases, we have generated transgenic mice that lack the mMCP-1 gene. They were backcrossed onto a congenic BALB/c background to investigate the response to nematode infection. The deletion of the mMCP-1 gene is associated with significantly delayed expulsion of Trichinella spiralis and increased deposition of muscle larvae in BALB/c mice despite the presence of normal and sometimes increased numbers of MMCs. Neither worm fecundity nor worm burdens were altered in Nippostrongylus-infected mMCP-1(-/)- BALB/c mice. These data demonstrate, for the first time, that the ablation of an MMC-derived effector molecule compromises the expulsion process.

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