Initiation of in Vitro MRNA Synthesis from the Wild-type Lac Promoter

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1975 Nov 1

PMID 1060117

Citations 32

Authors

J Majors

Affiliations

Soon will be listed here.

Abstract

An in vitro transcription system, dependent on catabolite gene activator protein (CAP), utilizing a 200 base-pair restriction fragment, has been used to show that the initiation site of the wild-type Escherichia coli lac mRNA, and that of two mutants, 8d and ps, are identical to that previously reported for the CAP-independent promoter mutant UV5. Order of addition experiments are used to show that the binding of lac repressor to the operator is competitive with that of the RNA polymerase (nucleosidetriphosphate:RNA nucleotidyltransferase, EC 2.7.7.6) to the promoter, thus demonstrating functional overlap of the operator and promoter sites.

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