Alterations in Two Conserved Regions of Promoter Sequence Lead to Altered Rates of Polymerase Binding and Levels of Gene Expression

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1980 Jun 25

PMID 6253889

Citations 10

Authors

J E Stefano

J W Ackerson

J D Gralla

Affiliations

Soon will be listed here.

Abstract

Characterization of recombinant lac promoters highlights the importance of two regions of sequence conservation in promoters. The "Pribnow box" sequences are necessary for specific transcription in this system. This specificity is maintained when a mutated upstream sequence is introduced. However, changing the upstream DNA sequences influences both the rate of RNA polymerase binding in vitro and levels of expression in vivo.

Citing Articles

The context of gene expression regulation.

Gibcus J, Dekker J F1000 Biol Rep. 2012; 4:8.

PMID: 22500194 PMC: 3318259. DOI: 10.3410/B4-8.

Transcription initiation by mix and match elements: flexibility for polymerase binding to bacterial promoters.

Hook-Barnard I, Hinton D Gene Regul Syst Bio. 2009; 1:275-93.

PMID: 19119427 PMC: 2613000.

Escherichia coli promoter opening and -10 recognition: mutational analysis of sigma70.

Fenton M, Lee S, Gralla J EMBO J. 2000; 19(5):1130-7.

PMID: 10698953 PMC: 305651. DOI: 10.1093/emboj/19.5.1130.

Segment-specific mutagenesis: extensive mutagenesis of a lac promoter/operator element.

Weiher H, Schaller H Proc Natl Acad Sci U S A. 1982; 79(5):1408-12.

PMID: 7041119 PMC: 345982. DOI: 10.1073/pnas.79.5.1408.

Spacer mutations in the lac ps promoter.

Stefano J, Gralla J Proc Natl Acad Sci U S A. 1982; 79(4):1069-72.

PMID: 6951162 PMC: 345901. DOI: 10.1073/pnas.79.4.1069.

References

Silverstone A, ARDITTI R, MAGASANIK B . Catabolite-insensitive revertants of lac promoter mutants. Proc Natl Acad Sci U S A. 1970; 66(3):773-9. PMC: 283117. DOI: 10.1073/pnas.66.3.773. View

de Crombrugghe B, Chen B, Gottesman M, Pastan I, Varmus H, Emmer M . Regulation of lac mRNA synthesis in a soluble cell-free system. Nat New Biol. 1971; 230(10):37-40. DOI: 10.1038/newbio230037a0. View

Beckwith J, Grodzicker T, Arditti R . Evidence for two sites in the lac promoter region. J Mol Biol. 1972; 69(1):155-60. DOI: 10.1016/0022-2836(72)90031-9. View

HINKLE D, CHAMBERLIN M . Studies of the binding of Escherichia coli RNA polymerase to DNA. II. The kinetics of the binding reaction. J Mol Biol. 1972; 70(2):187-95. DOI: 10.1016/0022-2836(72)90532-3. View

Maizels N . The nucleotide sequence of the lactose messenger ribonucleic acid transcribed from the UV5 promoter mutant of Escherichia coli. Proc Natl Acad Sci U S A. 1973; 70(12):3585-9. PMC: 427285. DOI: 10.1073/pnas.70.12.3585. View

Reznikoff W, Winter R, Hurley C . The location of the repressor binding sites in the lac operon. Proc Natl Acad Sci U S A. 1974; 71(6):2314-8. PMC: 388443. DOI: 10.1073/pnas.71.6.2314. View

CHAMBERLIN M . The selectivity of transcription. Annu Rev Biochem. 1974; 43(0):721-75. DOI: 10.1146/annurev.bi.43.070174.003445. View

Hopkins J . A new class of promoter mutations in the lactose operon of Escherichia coli. J Mol Biol. 1974; 87(4):715-24. DOI: 10.1016/0022-2836(74)90080-1. View

Dickson R, Abelson J, Barnes W, Reznikoff W . Genetic regulation: the Lac control region. Science. 1975; 187(4171):27-35. DOI: 10.1126/science.1088926. View

10.

Schaller H, Gray C, Herrmann K . Nucleotide sequence of an RNA polymerase binding site from the DNA of bacteriophage fd. Proc Natl Acad Sci U S A. 1975; 72(2):737-41. PMC: 432391. DOI: 10.1073/pnas.72.2.737. View

11.

Maniatis T, Jeffrey A, van deSande H . Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis. Biochemistry. 1975; 14(17):3787-94. DOI: 10.1021/bi00688a010. View

12.

Burgess R, Jendrisak J . A procedure for the rapid, large-scall purification of Escherichia coli DNA-dependent RNA polymerase involving Polymin P precipitation and DNA-cellulose chromatography. Biochemistry. 1975; 14(21):4634-8. DOI: 10.1021/bi00692a011. View

13.

Majors J . Initiation of in vitro mRNA synthesis from the wild-type lac promoter. Proc Natl Acad Sci U S A. 1975; 72(11):4394-8. PMC: 388728. DOI: 10.1073/pnas.72.11.4394. View

14.

Seeburg P, Nusslein C, Schaller H . Interaction of RNA polymerase with promoters from bacteriophage fd. Eur J Biochem. 1977; 74(1):107-13. DOI: 10.1111/j.1432-1033.1977.tb11372.x. View

15.

Dickson R, Abelson J, Johnson P . Nucleotide sequence changes produced by mutations in the lac promoter of Escherichia coli. J Mol Biol. 1977; 111(1):65-75. DOI: 10.1016/s0022-2836(77)80132-0. View

16.

Tait R, Rodriguez R, Boyer H . Altered tetracycline resistance in pSC101 recombinant plasmids. Mol Gen Genet. 1977; 151(3):327-31. DOI: 10.1007/BF00268797. View

17.

Widera G, Gautier F, Lindenmaier W, Collins J . The expression of tetracycline resistance after insertion of foreign DNA fragments between the EcoRI and HindIII sites of the plasmid cloning vector pBR 322. Mol Gen Genet. 1978; 163(3):301-5. DOI: 10.1007/BF00271959. View

18.

Scherer G, Walkinshaw M, Arnott S . A computer aided oligonucleotide analysis provides a model sequence for RNA polymerase-promoter recognition in E.coli. Nucleic Acids Res. 1978; 5(10):3759-73. PMC: 342708. DOI: 10.1093/nar/5.10.3759. View

19.

Maquat L, Reznikoff W . In vitro analysis of the Escherichia coli RNA polymerase interaction with wild-type and mutant lactose promoters. J Mol Biol. 1978; 125(4):467-90. DOI: 10.1016/0022-2836(78)90311-x. View

20.

Neve R, West R, Rodriguez R . Eukaryotic DNA fragments which act as promoters for a plasmid gene. Nature. 1979; 277(5694):324-5. DOI: 10.1038/277324a0. View