The Generation of a ColE1-Apr Cloning Vehicle Which Allows Detection of Inserted DNA

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1975 Dec 30

PMID 1045010

Citations 136

Authors

M So

R Gill

S Falkow

Affiliations

Soon will be listed here.

Abstract

A 3.2 Mdal sequence of DNA, TnA, which contains the ampicillin (Ap) resistance determinant has been translocated from an R plasmid to the plasmid ColE1. A total of 12 isolates were studied. There are at least 8 sites in ColE1 at which TnA has inserted. Insertion at five of these has resulted in a Col-phenotype. One ColE1-Apr plasmid, RSF2124, was examined further and its replication properties are found to be similar to that of the parent plasmid. RSF2124 appears to be a useful plasmid vehicle for the molecular cloning of DNA from diverse prokaryotic sources: it codes for readily detectable Ap resistance and contains a single EcoRI site in a gene affecting colicin biosynthesis so that it is unable to produce colicin upon ligation to other DNA.

Citing Articles

Natural and Synthetic Suppressor Mutations Defy Stability-Activity Tradeoffs.

Lee S, Okoye C, Biesbrock D, Harris E, Miyasaki K, Rilinger R Biochemistry. 2022; 61(5):398-407.

PMID: 35142509 PMC: 8893143. DOI: 10.1021/acs.biochem.1c00805.

Corrected Version Distribution of Heterogeneous and Homologous Plasmids in Bacillus spp.

Yoshimura K, Yamamoto O, Seki T, Oshima Y Appl Environ Microbiol. 1983; 46(6):1268-75.

PMID: 16346439 PMC: 239562. DOI: 10.1128/aem.46.6.1268-1275.1983.

Binding between the par region of plasmids R1 and pSC101 and the outer membrane fraction of the host bacteria.

Gustafsson P, Wolf-Watz H, Lind L, Johansson K, Nordstrom K EMBO J. 1983; 2(1):27-32.

PMID: 11894904 PMC: 555081. DOI: 10.1002/j.1460-2075.1983.tb01375.x.

A ColE1-encoded gene directs entry exclusion of the plasmid.

Yamada Y, Yamada M, Nakazawa A J Bacteriol. 1995; 177(21):6064-8.

PMID: 7592368 PMC: 177443. DOI: 10.1128/jb.177.21.6064-6068.1995.

Self-assembly of single and closely spaced nucleosome core particles.

Noll M, Zimmer S, Engel A, Dubochet J Nucleic Acids Res. 1980; 8(1):21-42.

PMID: 7355128 PMC: 327240. DOI: 10.1093/nar/8.1.21.

References

Cohen S, Chang A, Boyer H, Helling R . Construction of biologically functional bacterial plasmids in vitro. Proc Natl Acad Sci U S A. 1973; 70(11):3240-4. PMC: 427208. DOI: 10.1073/pnas.70.11.3240. View

Chang A, Cohen S . Genome construction between bacterial species in vitro: replication and expression of Staphylococcus plasmid genes in Escherichia coli. Proc Natl Acad Sci U S A. 1974; 71(4):1030-4. PMC: 388155. DOI: 10.1073/pnas.71.4.1030. View

Berg P, Baltimore D, Brenner S, Roblin R, Singer M . Summary statement of the Asilomar conference on recombinant DNA molecules. Proc Natl Acad Sci U S A. 1975; 72(6):1981-4. PMC: 432675. DOI: 10.1073/pnas.72.6.1981. View

Heffron F, Rubens C, Falkow S . Translocation of a plasmid DNA sequence which mediates ampicillin resistance: molecular nature and specificity of insertion. Proc Natl Acad Sci U S A. 1975; 72(9):3623-7. PMC: 433048. DOI: 10.1073/pnas.72.9.3623. View

Clewell D . Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol. J Bacteriol. 1972; 110(2):667-76. PMC: 247463. DOI: 10.1128/jb.110.2.667-676.1972. View

Bazaral M, Helinski D . Replication of a bacterial plasmid and an episome in Escherichia coli. Biochemistry. 1970; 9(2):399-406. DOI: 10.1021/bi00804a029. View

Kleckner N, Chan R, Tye B, Botstein D . Mutagenesis by insertion of a drug-resistance element carrying an inverted repetition. J Mol Biol. 1975; 97(4):561-75. DOI: 10.1016/s0022-2836(75)80059-3. View

Lai C, Nathans D . Deletion mutants of simian virus 40 generated by enzymatic excision of DNA segments from the viral genome. J Mol Biol. 1974; 89(1):179-93. DOI: 10.1016/0022-2836(74)90169-7. View

Cohen S, Chang A, Hsu L . Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA. Proc Natl Acad Sci U S A. 1972; 69(8):2110-4. PMC: 426879. DOI: 10.1073/pnas.69.8.2110. View

10.

Berg D, Davies J, Allet B, Rochaix J . Transposition of R factor genes to bacteriophage lambda. Proc Natl Acad Sci U S A. 1975; 72(9):3628-32. PMC: 433049. DOI: 10.1073/pnas.72.9.3628. View

11.

Bazaral M, Helinski D . Characterization of multiple circular DNA forms of colicinogenic factor E-1 from Proteus mirabilis. Biochemistry. 1968; 7(10):3513-20. DOI: 10.1021/bi00850a028. View

12.

Siccardi A . Colicin resistance associated with resistance factors in Escherichia coli. Genet Res. 1966; 8(2):219-28. DOI: 10.1017/s0016672300010077. View

13.

Pritchard R, Zaritsky A . Effect of thymine concentration on the replication velocity of DNA in a thymineless mutant of Escherichia coli. Nature. 1970; 226(5241):126-31. DOI: 10.1038/226126a0. View

14.

MEYNELL E, Meynell G, Datta N . Phylogenetic relationships of drug-resistance factors and other transmissible bacterial plasmids. Bacteriol Rev. 1968; 32(1):55-83. PMC: 378292. DOI: 10.1128/br.32.1.55-83.1968. View

15.

Heffron F, Sublett R, Hedges R, Jacob A, Falkow S . Origin of the TEM-beta-lactamase gene found on plasmids. J Bacteriol. 1975; 122(1):250-6. PMC: 235664. DOI: 10.1128/jb.122.1.250-256.1975. View

16.

Murray N, Murray K . Manipulation of restriction targets in phage lambda to form receptor chromosomes for DNA fragments. Nature. 1974; 251(5475):476-81. DOI: 10.1038/251476a0. View

17.

Kedes L, Chang A, Houseman D, Cohen S . Isolation of histone genes from unfractionated sea urchin DNA by subculture cloning in E. coli. Nature. 1975; 255(5509):533-8. DOI: 10.1038/255533a0. View

18.

Helinski D, Clewell D . Circular DNA. Annu Rev Biochem. 1971; 40:899-942. DOI: 10.1146/annurev.bi.40.070171.004343. View

19.

Tanaka T, Weisblum B . Construction of a colicin E1-R factor composite plasmid in vitro: means for amplification of deoxyribonucleic acid. J Bacteriol. 1975; 121(1):354-62. PMC: 285650. DOI: 10.1128/jb.121.1.354-362.1975. View

20.

Morrow J, Cohen S, Chang A, Boyer H, Goodman H, Helling R . Replication and transcription of eukaryotic DNA in Escherichia coli. Proc Natl Acad Sci U S A. 1974; 71(5):1743-7. PMC: 388315. DOI: 10.1073/pnas.71.5.1743. View