Genome Construction Between Bacterial Species in Vitro: Replication and Expression of Staphylococcus Plasmid Genes in Escherichia Coli

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1974 Apr 1

PMID 4598290

Citations 62

Authors

A C Chang

S N Cohen

Affiliations

Soon will be listed here.

Abstract

Genes carried by EcoRI endonuclease-generated fragments of Staphylococcus plasmid DNA have been covalently joined to the E. coli antibiotic-resistance plasmid pSC101, and the resulting hybrid molecules have been introduced into E. coli by transformation. The newly constructed plasmids replicate as biologically functional units in E. coli, and express genetic information carried by both of the parent DNA molecules. In addition, electron microscope heteroduplex analysis of the recombinant plasmids indicate that they contain DNA sequences derived from E. coli and Staphylococcus aureus. Recombinant molecules can transform other E. coli cells for penicillin-resistance markers originally carried by the staphylococcal plasmid, and can be transferred among E. coli strains by conjugally proficient transfer plasmids.

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