Tetracycline Resistance Genes from Bacillus Plasmid PAB124 Confer Decreased Accumulation of the Antibiotic in Bacillus Subtilis but Not in Escherichia Coli

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1981 Mar 1

PMID 6782092

Citations 12

Authors

S Eccles

A Docherty

I Chopra

S Shales

P Ball

Affiliations

Soon will be listed here.

Abstract

Expression of tetracycline resistance by genes originating in the Bacillus plasmid pAB124 was examined in both Bacillus subtilis and Escherichia coli host cells. Expression of resistance in B. subtilis by genes from pAB124 was inducible and associated with decreased accumulation of the antibiotic. A fragment of pAB124 carrying the genes coding for tetracycline resistance was cloned into the E. coli plasmid RSF2124. The cloned fragment conferred a low level of resistance in E. coli, but this was not associated with decreased uptake of tetracycline and was not inducible.

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References

Anagnostopoulos C, Spizizen J . REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS. J Bacteriol. 1961; 81(5):741-6. PMC: 279084. DOI: 10.1128/jb.81.5.741-746.1961. View

Mendez B, Tachibana C, Levy S . Heterogeneity of tetracycline resistance determinants. Plasmid. 1980; 3(2):99-108. DOI: 10.1016/0147-619x(80)90101-8. View

So M, Gill R, Falkow S . The generation of a ColE1-Apr cloning vehicle which allows detection of inserted DNA. Mol Gen Genet. 1975; 142(3):239-49. DOI: 10.1007/BF00425649. View

Thuring R, Sanders J, Borst P . A freeze-squeeze method for recovering long DNA from agarose gels. Anal Biochem. 1975; 66(1):213-20. DOI: 10.1016/0003-2697(75)90739-3. View

Chopra I, Howe T . Bacterial resistance to the tetracyclines. Microbiol Rev. 1978; 42(4):707-24. PMC: 281453. DOI: 10.1128/mr.42.4.707-724.1978. View

Bingham A, Bruton C, Atkinson T . Characterization of Bacillus stearothermophilus plasmid pab124 and construction of deletion variants. J Gen Microbiol. 1980; 119(1):109-15. DOI: 10.1099/00221287-119-1-109. View

Shales S, Chopra I, Ball P . Evidence for more than one mechanism of plasmid-determined tetracycline resistance in Escherichia coli. J Gen Microbiol. 1980; 121(1):221-9. DOI: 10.1099/00221287-121-1-221. View

Bingham A, Bruton C, Atkinson T . Isolation and partial characterization of four plasmids from antibiotic-resistant thermophilic bacilli. J Gen Microbiol. 1979; 114(2):401-8. DOI: 10.1099/00221287-114-2-401. View

Chen C, Thomas Jr C . Recovery of DNA segments from agarose gels. Anal Biochem. 1980; 101(2):339-41. DOI: 10.1016/0003-2697(80)90197-9. View

10.

Cooper S, Helmstetter C . Chromosome replication and the division cycle of Escherichia coli B/r. J Mol Biol. 1968; 31(3):519-40. DOI: 10.1016/0022-2836(68)90425-7. View

11.

Chopra I, Eccles S . Diffusion of tetracycline across the outer membrane of Escherichia coli K-12: involvement of protein Ia. Biochem Biophys Res Commun. 1978; 83(2):550-7. DOI: 10.1016/0006-291x(78)91025-2. View

12.

Ball P, Shales S, Chopra I . Plasmid-mediated tetracycline resistance in Escherichia coli involves increased efflux of the antibiotic. Biochem Biophys Res Commun. 1980; 93(1):74-81. DOI: 10.1016/s0006-291x(80)80247-6. View

13.

Shivakumar A, Dubnau D . Plasmid replication in DNA Ts mutants of Bacillus subtilis. Plasmid. 1978; 1(3):405-16. DOI: 10.1016/0147-619x(78)90055-0. View

14.

Wake R . Circularity of the Bacillus subtilis chromosome and further studies on its bidirectional replication. J Mol Biol. 1973; 77(4):569-75. DOI: 10.1016/0022-2836(73)90223-4. View

15.

Ward J, Grinsted J . Mapping of functions in the R-plasmid R388 by examination of deletion mutants generated in vitro. Gene. 1978; 3(2):87-95. DOI: 10.1016/0378-1119(78)90053-7. View

16.

McMurry L, Petrucci Jr R, Levy S . Active efflux of tetracycline encoded by four genetically different tetracycline resistance determinants in Escherichia coli. Proc Natl Acad Sci U S A. 1980; 77(7):3974-7. PMC: 349750. DOI: 10.1073/pnas.77.7.3974. View