An Unusual RNA Polymerase Binding Site in the Immunity Region of Phage Lambda

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1980 Jan 1

PMID 6450873

Citations 7

Authors

V Pirrotta

K. Ineichen

A Walz

Affiliations

Soon will be listed here.

Abstract

RNA polymerase binds very tightly at a site called Brex in the lambda immunity region, to the left of the rex gene and about 600 nucleotides to the right of PL. The complex formed is resistant to 1 M NaCl in the absence of nucleotide triphosphate. While in vitro little or no transcription is observed from Brex, in vivo, when inserted in a plasmid vector which allows detection of its activity, it acts as an efficient promoter. We have mapped the site protected by RNA polymerase against DNase and determined its sequence which is abnormal compared that of an average promoter.

Citing Articles

Study of Ren, RexA, and RexB Functions Provides Insight Into the Complex Interaction Between Bacteriophage λ and Its Host, .

Thomason L, Court D Phage (New Rochelle). 2022; 3(3):153-164.

PMID: 36204488 PMC: 9529316. DOI: 10.1089/phage.2022.0020.

Elements in the λ immunity region regulate phage development: beyond the 'Genetic Switch'.

Thomason L, Morrill K, Murray G, Court C, Shafer B, Schneider T Mol Microbiol. 2019; 112(6):1798-1813.

PMID: 31545538 PMC: 8103288. DOI: 10.1111/mmi.14394.

Enhanced expression of cro-beta-galactosidase fusion proteins under the control of the PR promoter of bacteriophage lambda.

Zabeau M, Stanley K EMBO J. 1982; 1(10):1217-24.

PMID: 6327257 PMC: 553192. DOI: 10.1002/j.1460-2075.1982.tb00016.x.

Restriction endonuclease EcaI from Enterobacter cloacae.

HOBOM G, Schwarz E, Melzer M, Mayer H Nucleic Acids Res. 1981; 9(19):4823-32.

PMID: 6273787 PMC: 327482. DOI: 10.1093/nar/9.19.4823.

The rex gene of bacteriophage lambda is really two genes.

Matz K, Schmandt M, Gussin G Genetics. 1982; 102(3):319-27.

PMID: 6217105 PMC: 1201943. DOI: 10.1093/genetics/102.3.319.

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