Yusuke Kamachi
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Explore the profile of Yusuke Kamachi including associated specialties, affiliations and a list of published articles.
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31
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1288
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Recent Articles
1.
Aoki K, Yamasaki M, Umezono R, Hamamoto T, Kamachi Y
Cells
. 2024 Feb;
13(3.
PMID: 38334653
Successful genome editing depends on the cleavage efficiency of programmable nucleases (PNs) such as the CRISPR-Cas system. Various methods have been developed to assess the efficiency of PNs, most of...
2.
Kamachi Y, Kawahara A
Methods Mol Biol
. 2023 Feb;
2637:313-324.
PMID: 36773157
CRISPR-Cas9 genome editing technology has been successfully applied to generate various genetic modifications in zebrafish. The CRISPR-Cas9 system, which originally consisted of three components, CRISPR RNA (crRNA), trans-activating crRNA (tracrRNA),...
3.
Okada K, Aoki K, Tabei T, Sugio K, Imai K, Bonkohara Y, et al.
Nucleic Acids Res
. 2022 Feb;
50(5):2854-2871.
PMID: 35166844
Specific sequence features of the protospacer and protospacer-adjacent motif (PAM) are critical for efficient cleavage by CRISPR-Cas9, but current knowledge is largely derived from single-guide RNA (sgRNA) systems assessed in...
4.
Ranawakage D, Okada K, Sugio K, Kawaguchi Y, Kuninobu-Bonkohara Y, Takada T, et al.
Front Cell Dev Biol
. 2021 Mar;
8:598634.
PMID: 33681181
Despite the unprecedented gene editing capability of CRISPR-Cas9-mediated targeted knock-in, the efficiency and precision of this technology still require further optimization, particularly for multicellular model organisms, such as the zebrafish...
5.
Ranawakage D, Takada T, Kamachi Y
Sci Rep
. 2019 May;
9(1):6895.
PMID: 31053795
The affinity of an antibody for its antigen serves as a critical parameter for antibody evaluation. The evaluation of antibody-antigen affinity is essential for a successful antibody-based assay, particularly immunoprecipitation...
6.
Okamoto Y, Nishimura N, Matsuda K, Ranawakage D, Kamachi Y, Kondoh H, et al.
Dev Growth Differ
. 2018 Mar;
60(3):133-145.
PMID: 29520762
To elucidate the transcriptional regulation that underlies specification of the otic placode, we investigated the Sox3 downstream enhancer Otic1 of the chicken, the activity of which is restricted to and...
7.
Yasumi T, Inoue M, Maruhashi M, Kamachi Y, Higashi Y, Kondoh H, et al.
Dev Growth Differ
. 2015 Dec;
58(2):205-14.
PMID: 26691438
The vertebrate Zfhx1 transcription factor family comprises δEF1 and Sip1, which bind to CACCT-containing sequences and act as transcriptional repressors. It has been a longstanding question whether these transcription factors...
8.
Morimura H, Tanaka S, Ishitobi H, Mikami T, Kamachi Y, Kondoh H, et al.
ACS Nano
. 2013 Nov;
7(12):10733-40.
PMID: 24195575
The plasmon resonant wavelength for a pair of gold nanoparticles, or gold nanodimer, increases inversely with the gap distance between the two nanoparticles. Taking advantage of this property, we performed...
9.
Kamachi Y, Kondoh H
Development
. 2013 Oct;
140(20):4129-44.
PMID: 24086078
Sox transcription factors play widespread roles during development; however, their versatile funtions have a relatively simple basis: the binding of a Sox protein alone to DNA does not elicit transcriptional...
10.
Kobayashi I, Kojima K, Uchino K, Sezutsu H, Iizuka T, Tatematsu K, et al.
Arch Insect Biochem Physiol
. 2011 Jan;
76(4):195-210.
PMID: 21254202
A binary gene expression system using the yeast GAL4 DNA-binding protein and the upstream activating sequence (UAS) of galactose-driven yeast genes is an established and powerful tool for the analysis...