Wakako Tsugawa
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Explore the profile of Wakako Tsugawa including associated specialties, affiliations and a list of published articles.
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Articles
65
Citations
429
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Recent Articles
1.
Miura D, Hayashi W, Hirano K, Sasaki I, Tsukakoshi K, Kakizoe H, et al.
Adv Sci (Weinh)
. 2024 Sep;
11(43):e2403871.
PMID: 39316377
The SARS-CoV-2 pandemic has challenged more scientists to detect viruses and to visualize virus-containing spots for diagnosis and infection control; however, detection principles of commercially available technologies are not optimal...
2.
Hoshi K, Tu A, Shobo M, Kettisen K, Ye L, Bulow L, et al.
Nanomaterials (Basel)
. 2024 Sep;
14(17).
PMID: 39269102
Hemozoin (Hz) is a heme crystal produced during malaria infection that stimulates immune cells, leading to the production of cytokines and chemokines. The immunostimulatory action of Hz has previously been...
3.
Oda M, Hiraka K, Tsugawa W, Ikebukuro K, Sode K, Asano R
Biosens Bioelectron
. 2024 Sep;
267:116741.
PMID: 39250870
Antibody-enzyme complexes (AECs) are ideal for immunosensing. Although AECs using antibody fragments can be produced by bacterial hosts, their low affinity limits their sensing applications. We have improved the affinity...
4.
Ikegai K, Okuda-Shimazaki J, Tran T, Hatada M, Asano R, Ikebukuro K, et al.
Biosens Bioelectron
. 2024 Mar;
255:116219.
PMID: 38552525
We introduce a versatile method to convert NAD or NADP -dependent dehydrogenases into quasi-direct electron transfer (quasi-DET)-type dehydrogenases, by modifying with a mediator on the enzyme surface toward the development...
5.
Miura D, Motohashi S, Goto A, Kimura H, Tsugawa W, Sode K, et al.
Int J Mol Sci
. 2024 Mar;
25(5).
PMID: 38474105
Although IgG-free immunosensors are in high demand owing to ethical concerns, the development of convenient immunosensors that alternatively integrate recombinantly produced antibody fragments, such as single-chain variable fragments (scFvs), remains...
6.
Komiya E, Takamatsu S, Miura D, Tsukakoshi K, Tsugawa W, Sode K, et al.
J Am Chem Soc
. 2024 Jan;
146(6):4087-4097.
PMID: 38295327
DNA-protein complexes are attractive components with broad applications in various research fields, such as DNA aptamer-enzyme complexes as biosensing elements. However, noncovalent DNA-protein complexes often decrease detection sensitivity because they...
7.
Yanase T, Okuda-Shimazaki J, Asano R, Ikebukuro K, Sode K, Tsugawa W
Int J Mol Sci
. 2023 Feb;
24(3).
PMID: 36768169
The electrochemical enzyme sensors based on direct electron transfer (DET)-type oxidoreductase-based enzymes are ideal for continuous and in vivo monitoring. However, the number and types of DET-type oxidoreductases are limited....
8.
Okuda-Shimazaki J, Yoshida H, Lee I, Kojima K, Suzuki N, Tsugawa W, et al.
Commun Biol
. 2022 Dec;
5(1):1334.
PMID: 36473944
The heterotrimeric flavin adenine dinucleotide dependent glucose dehydrogenase is a promising enzyme for direct electron transfer (DET) principle-based glucose sensors within continuous glucose monitoring systems. We elucidate the structure of...
9.
Hiraka K, Yoshida H, Tsugawa W, Asano R, La Belle J, Ikebukuro K, et al.
Protein Sci
. 2022 Sep;
31(10):e4434.
PMID: 36173159
l-Lactate oxidase (LOx) is a flavin mononucleotide (FMN)-dependent triose phosphate isomerase (TIM) barrel fold enzyme that catalyzes the oxidation of l-lactate using oxygen as a primary electron acceptor. Although reductive...
10.
Takamatsu S, Lee I, Lee J, Asano R, Tsugawa W, Ikebukuro K, et al.
Biosens Bioelectron
. 2022 Jan;
200:113927.
PMID: 34995837
d-Serine biosensing has been extensively reported based on enzyme sensors using flavin adenine dinucleotide (FAD) -dependent d-amino acid oxidase (DAAOx), based on the monitoring of hydrogen peroxide generated by the...