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Wakako Tsugawa

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Articles 65
Citations 429
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Recent Articles
11.
Miura D, Kimura H, Tsugawa W, Ikebukuro K, Sode K, Asano R
Talanta . 2021 Aug; 234:122638. PMID: 34364447
Human hemoglobin (Hb) is a biomarker of several diseases, and monitoring of Hb levels is required during emergent surgery. However, rapid and sensitive Hb detection methods are yet to be...
12.
Hatada M, Loew N, Okuda-Shimazaki J, Khanwalker M, Tsugawa W, Mulchandani A, et al.
Molecules . 2021 Feb; 26(3). PMID: 33572552
Glycated albumin (GA) is an important glycemic control marker for diabetes mellitus. This study aimed to develop a highly sensitive disposable enzyme sensor strip for GA measurement by using an...
13.
Hatada M, Saito S, Yonehara S, Tsugawa W, Asano R, Ikebukuro K, et al.
Biosens Bioelectron . 2021 Jan; 177:112984. PMID: 33477030
Haemoglobin A1c (hemoglobin A1c, HbA1c) is an important long-term glycemic control marker for diabetes. The aim of this study was to develop an enzyme flow injection analysis (FIA) system using...
14.
Ito K, Okuda-Shimazaki J, Kojima K, Mori K, Tsugawa W, Asano R, et al.
Biosens Bioelectron . 2021 Jan; 176:112911. PMID: 33421758
A fusion enzyme composed of an Aspergillus flavus-derived flavin adenine dinucleotide glucose dehydrogenase (AfGDH) and an electron transfer domain of Phanerochaete chrysosporium-derived cellobiose dehydrogenase (Pcyb) was previously reported to show...
15.
Hiraka K, Tsugawa W, Asano R, Yokus M, Ikebukuro K, Daniele M, et al.
Biosens Bioelectron . 2021 Jan; 176:112933. PMID: 33395570
The development of wearable multiplexed biosensors has been focused on systems to measure sweat l-lactate and other metabolites, where the employment of the direct electron transfer (DET) principle is expected....
16.
Kimura H, Miura D, Tsugawa W, Ikebukuro K, Sode K, Asano R
Biosens Bioelectron . 2020 Dec; 175:112885. PMID: 33342691
Antibody-enzyme complexes (AECs) with binding ability to specific targets and catalytic activities to gain signals are known to be ideal sensing elements; however, AEC-based universal sensors applicable to point-of-care testing...
17.
Yanase T, Okuda-Shimazaki J, Mori K, Kojima K, Tsugawa W, Sode K
Biochem Biophys Res Commun . 2020 Aug; 530(1):82-86. PMID: 32828319
Fungi-derived flavin adenine dinucleotide (FAD)-dependent glucose dehydrogenases (FADGDHs) are the most popular and advanced enzymes for SMBG sensors because of their high substrate specificity toward glucose and oxygen insensitivity. However,...
18.
Hiraka K, Tsugawa W, Sode K
Int J Mol Sci . 2020 May; 21(11). PMID: 32471202
In this review, recent progress in the engineering of the oxidative half-reaction of flavin-dependent oxidases and dehydrogenases is discussed, considering their current and future applications in bioelectrochemical studies, such as...
19.
Fitriana M, Loew N, Witarto A, Ikebukuro K, Sode K, Tsugawa W
Sensors (Basel) . 2020 May; 20(10). PMID: 32429321
In this paper, a novel electron mediator, 1-methoxy-5-ethyl phenazinium ethyl sulfate (mPES), was introduced as a versatile mediator for disposable enzyme sensor strips, employing representative flavin oxidoreductases, lactate oxidase (LOx),...
20.
Suzuki N, Lee J, Loew N, Takahashi-Inose Y, Okuda-Shimazaki J, Kojima K, et al.
Int J Mol Sci . 2020 Feb; 21(3). PMID: 32046321
Glucose oxidase (GOx) has been widely utilized for monitoring glycemic levels due to its availability, high activity, and specificity toward glucose. Among the three generations of electrochemical glucose sensor principles,...