Sylvie Perret
Overview
Explore the profile of Sylvie Perret including associated specialties, affiliations and a list of published articles.
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15
Citations
684
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Recent Articles
1.
Delafosse L, Lord-Dufour S, Pelletier A, Perret S, Burlacu A, Ouimet M, et al.
Methods Mol Biol
. 2024 Jun;
2810:99-121.
PMID: 38926275
The continuous improvement of expression platforms is necessary to respond to the increasing demand for recombinant proteins that are required to carry out structural or functional studies as well as...
2.
Sauvageau J, Koyuturk I, St Michael F, Brochu D, Goneau M, Schoenhofen I, et al.
Commun Chem
. 2023 Sep;
6(1):189.
PMID: 37684364
Glycosylation is a key quality attribute that must be closely monitored for protein therapeutics. Established assays such as HILIC-Fld of released glycans and LC-MS of glycopeptides work well for glycoproteins...
3.
Joubert S, Stuible M, Lord-Dufour S, Lamoureux L, Vaillancourt F, Perret S, et al.
Biotechnol Bioeng
. 2023 Mar;
120(7):1746-1761.
PMID: 36987713
Protein expression from stably transfected Chinese hamster ovary (CHO) clones is an established but time-consuming method for manufacturing therapeutic recombinant proteins. The use of faster, alternative approaches, such as non-clonal...
4.
Rossotti M, van Faassen H, Tran A, Sheff J, Sandhu J, Duque D, et al.
Commun Biol
. 2022 Sep;
5(1):933.
PMID: 36085335
Nanobodies offer several potential advantages over mAbs for the control of SARS-CoV-2. Their ability to access cryptic epitopes conserved across SARS-CoV-2 variants of concern (VoCs) and feasibility to engineer modular,...
5.
Joubert S, Guimond J, Perret S, Malenfant F, Elahi S, Marcil A, et al.
Biotechnol Bioeng
. 2022 May;
119(8):2206-2220.
PMID: 35509261
Some effector functions prompted by immunoglobulin G (IgG) antibodies, such as antibody-dependent cell-mediated cytotoxicity (ADCC), strongly depend on the N-glycans linked to asparagine 297 of the Fc region of the...
6.
Stuible M, Gervais C, Lord-Dufour S, Perret S, LAbbe D, Schrag J, et al.
J Biotechnol
. 2020 Dec;
326:21-27.
PMID: 33301853
Recombinant forms of the spike protein of SARS-CoV-2 and related viruses have proven difficult to produce with good yields in mammalian cells. Given the panoply of potential COVID-19 diagnostic tools...
7.
Stuible M, Burlacu A, Perret S, Brochu D, Paul-Roc B, Baardsnes J, et al.
J Biotechnol
. 2018 Jun;
281:39-47.
PMID: 29886030
For pre-clinical evaluation of biotherapeutic candidates, protein production by transient gene expression (TGE) in Chinese Hamster Ovary (CHO) cells offers important advantages, including the capability of rapidly and cost-effectively generating...
8.
Poulain A, Perret S, Malenfant F, Mullick A, Massie B, Durocher Y
J Biotechnol
. 2017 Jun;
255:16-27.
PMID: 28625678
To rapidly produce large amounts of recombinant proteins, the generation of stable Chinese Hamster Ovary (CHO) cell pools represents a useful alternative to large-scale transient gene expression (TGE). We have...
9.
Mignaqui A, Ruiz V, Perret S, St-Laurent G, Chahal P, Transfiguracion J, et al.
PLoS One
. 2013 Aug;
8(8):e72800.
PMID: 23977353
Foot-and-mouth disease (FMD) is a highly contagious disease of cloven-hoofed animals. It produces severe economic losses in the livestock industry. Currently available vaccines are based on inactivated FMD virus (FMDV)....
10.
Agrawal V, Slivac I, Perret S, Bisson L, St-Laurent G, Murad Y, et al.
Methods Mol Biol
. 2012 Aug;
911:287-303.
PMID: 22886259
Camelid single domain antibodies fused to noncamelid Fc regions, also called chimeric heavy chain antibodies (cHCAb), offer great potential as therapeutic and diagnostic candidates due to their relatively small size...