Stephen P Perfetto
Overview
Explore the profile of Stephen P Perfetto including associated specialties, affiliations and a list of published articles.
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29
Citations
1375
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Recent Articles
1.
Reifel K, Swan B, Jellison E, Ambrozak D, Baijer J, Nguyen R, et al.
Cytometry A
. 2020 Jun;
97(7):674-680.
PMID: 32488957
In response to the recent COVID-19 pandemic, many laboratories are involved in research supporting SARS-CoV-2 vaccine development and clinical trials. Flow cytometry laboratories will be responsible for a large part...
2.
Morales-Kastresana A, Musich T, Welsh J, Telford W, Demberg T, Wood J, et al.
J Extracell Vesicles
. 2019 Jul;
8(1):1597603.
PMID: 31258878
Biological nanoparticles, including viruses and extracellular vesicles (EVs), are of interest to many fields of medicine as biomarkers and mediators of or treatments for disease. However, exosomes and small viruses...
3.
Perfetto S, Hogarth P, Monard S, Fontes B, Reifel K, Swan B, et al.
Cytometry A
. 2018 Dec;
95(2):173-182.
PMID: 30561906
Today's state-of-the-art cell sorting flow cytometers are equipped with aerosol containment systems designed to evacuate aerosols from the sort chamber during a sort. This biosafety device is especially important when...
4.
Parks D, Moore W, Brinkman R, Chen Y, Condello D, El Khettabi F, et al.
Cytometry A
. 2018 Sep;
93(11):1087-1091.
PMID: 30244531
We demonstrate improved methods for making valid and accurate comparisons of fluorescence measurement capabilities among instruments tested at different sites and times. We designed a suite of measurements and automated...
5.
Parks D, El Khettabi F, Chase E, Hoffman R, Perfetto S, Spidlen J, et al.
Cytometry A
. 2017 Feb;
91(3):232-249.
PMID: 28160404
We developed a fully automated procedure for analyzing data from LED pulses and multilevel bead sets to evaluate backgrounds and photoelectron scales of cytometer fluorescence channels. The method improves on...
6.
Perfetto S, Chattopadhyay P, Wood J, Nguyen R, Ambrozak D, Hill J, et al.
Cytometry A
. 2014 Oct;
85(12):1037-48.
PMID: 25346474
Much of the complexity of multicolor flow cytometry experiments lies within the development of antibody staining panels and the standardization of instruments. In this article, we propose a theoretical metric...
7.
Holmes K, Fontes B, Hogarth P, Konz R, Monard S, Pletcher Jr C, et al.
Cytometry A
. 2014 Mar;
85(5):434-53.
PMID: 24634405
Flow cytometric cell sorting of biological specimens has become prevalent in basic and clinical research laboratories. These specimens may contain known or unknown infectious agents, necessitating precautions to protect instrument...
8.
Perfetto S, Ambrozak D, Nguyen R, Chattopadhyay P, Roederer M
Nat Protoc
. 2012 Nov;
7(12):2067-79.
PMID: 23138348
The quality assurance program presented here provides a means to maximize and maintain the performance of individual flow cytometers in a facility. To optimize performance, we recommend performing all three...
9.
Zarkowsky D, Lamoreaux L, Chattopadhyay P, Koup R, Perfetto S, Roederer M
Cytometry A
. 2010 Dec;
79(1):84-9.
PMID: 21182185
Quantum dots (QD) are fluorescent nanocrystals that are highly useful in imaging and flow cytometric analyses. During routine use of monoclonal antibody conjugates of QD, we have occasionally seen partial...
10.
Perfetto S, Ambrozak D, Nguyen R, Roederer M, Koup R, Holmes K
Methods Mol Biol
. 2010 Dec;
699:449-69.
PMID: 21116997
Over the past decade, there has been a rapid growth in the number of BSL-3 and BSL-4 laboratories in the USA and an increase in demand for infectious cell sorting...