Stephen P Perfetto
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Explore the profile of Stephen P Perfetto including associated specialties, affiliations and a list of published articles.
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29
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1375
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Recent Articles
11.
Zhang H, Song K, Rabin R, Hill B, Perfetto S, Roederer M, et al.
J Immunol
. 2010 Oct;
185(11):6646-63.
PMID: 20980630
Because T cells act primarily through short-distance interactions, homing receptors can identify colocalizing cells that serve common functions. Expression patterns for multiple chemokine receptors on CD4(+) T cells from human...
12.
Chattopadhyay P, Perfetto S, Roederer M
Discov Med
. 2010 Aug;
4(23):255-62.
PMID: 20704956
Extract: Human blood contains hundreds of distinct cell types, which differ by the proteins they produce (including those expressed on the cell surface) and the functions they perform. Numerous clinical...
13.
Perfetto S, Chattopadhyay P, Lamoreaux L, Nguyen R, Ambrozak D, Koup R, et al.
Curr Protoc Cytom
. 2010 Jun;
Chapter 9:Unit 9.34.
PMID: 20578108
Amine-reactive dyes, also known as LIVE/DEAD fixable dead cell stains, are a class of viability dyes suitable for identifying dead cells in samples that will be fixed. These dyes cross...
14.
Chattopadhyay P, Perfetto S, Yu J, Roederer M
Wiley Interdiscip Rev Nanomed Nanobiotechnol
. 2010 Jan;
2(4):334-48.
PMID: 20101649
Because of their unique fluorescence properties, quantum dots (QDs) represent a promising new technology in the realm of multicolor flow cytometry. Although commercial reagents and applications for the technology are...
15.
Schmid I, Lambert C, Ambrozak D, Perfetto S
Curr Protoc Cytom
. 2008 Sep;
Chapter 3:Unit3.6.
PMID: 18770851
Cell sorting of viable biological specimens has become widespread in laboratories involved in basic and clinical research. As these samples can contain infectious agents, precautions to protect instrument operators and...
16.
Perfetto S, Ambrozak D, Nguyen R, Chattopadhyay P, Roederer M
Nat Protoc
. 2007 Apr;
1(3):1522-30.
PMID: 17406444
This protocol outlines a three-part quality assurance program to optimize, calibrate and monitor flow cytometers used to measure cells labeled with five or more fluorochromes (a practice known as polychromatic...
17.
Schmid I, Lambert C, Ambrozak D, Marti G, Moss D, Perfetto S
Cytometry A
. 2007 Mar;
71(6):414-37.
PMID: 17385740
Background: Cell sorting of viable biological specimens has become very prevalent in laboratories involved in basic and clinical research. As these samples can contain infectious agents, precautions to protect instrument...
18.
Perfetto S, Roederer M
Cytometry A
. 2007 Jan;
71(2):73-9.
PMID: 17200955
Objective: We evaluated the use of a high power, diode pulsed solid-state laser emitting 532 nm light for immunofluorescence applications. We compared the sensitivity and utility of this laser with...
19.
Chattopadhyay P, Price D, Harper T, Betts M, Yu J, Gostick E, et al.
Nat Med
. 2006 Jul;
12(8):972-7.
PMID: 16862156
Immune responses arise from a wide variety of cells expressing unique combinations of multiple cell-surface proteins. Detailed characterization is hampered, however, by limitations in available probes and instrumentation. Here, we...
20.
Perfetto S, Chattopadhyay P, Lamoreaux L, Nguyen R, Ambrozak D, Koup R, et al.
J Immunol Methods
. 2006 Jun;
313(1-2):199-208.
PMID: 16756987
Membrane-damaged cells caused by either mechanical trauma or through normal biological processes can produce artifacts in immunophenotyping analysis by flow cytometry. Dead cells can nonspecifically bind monoclonal antibody conjugates, potentially...