Stephen Bustin
Overview
Explore the profile of Stephen Bustin including associated specialties, affiliations and a list of published articles.
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23
Citations
360
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Recent Articles
1.
Untergasser A, Hellemans J, Pfaffl M, Ruijter J, van den Hoff M, Dragomir M, et al.
Mol Oncol
. 2023 Mar;
17(5):713-717.
PMID: 36916500
Accuracy and transparency of scientific data are becoming more and more relevant with the increasing concern regarding the evaluation of data reproducibility in many research areas. This concern is also...
2.
Bustin S, Bustin C, Kirvell S, Nolan T, Mueller R, Shipley G
Int J Mol Sci
. 2022 Aug;
23(15).
PMID: 35955620
The COVID-19 pandemic resulted in a universal, immediate, and vast demand for comprehensive molecular diagnostic testing, especially real-time quantitative (qPCR)-based methods. This rapidly triggered a global shortage of testing capacity,...
3.
Bustin S, Kirvell S, Huggett J, Nolan T
Int J Mol Sci
. 2021 Aug;
22(16).
PMID: 34445406
The reverse transcription quantitative polymerase chain reaction (RT-qPCR) is an established tool for the diagnosis of RNA pathogens. Its potential for automation has caused it to be used as a...
4.
Bustin S, Mueller R, Shipley G, Nolan T
Int J Mol Sci
. 2021 Mar;
22(5).
PMID: 33671091
Although molecular testing, and RT-qPCR in particular, has been an indispensable component in the scientific armoury targeting SARS-CoV-2, there are numerous falsehoods, misconceptions, assumptions and exaggerated expectations with regards to...
5.
Bustin S, Coward A, Sadler G, Teare L, Nolan T
Sci Rep
. 2020 Dec;
10(1):22214.
PMID: 33335187
Accurate, reliable and rapid detection of SARS-CoV-2 is essential not only for correct diagnosis of individual COVID-19 disease but also for the development of a rational strategy aimed at lifting...
6.
Sanders R, Bustin S, Huggett J, Mason D
Biomol Detect Quantif
. 2018 Jun;
15:13-17.
PMID: 29922589
Human health and safety depend on reliable measurements in medical diagnosis and on tests that support the selection and evaluation of therapeutic intervention and newly discovered molecular biomarkers must pass...
7.
Prattes J, Hoenigl M, Zinke S, Heldt S, Eigl S, Johnson G, et al.
Mycoses
. 2018 Feb;
61(6):355-359.
PMID: 29460450
The newly developed AspID PCR assay for detection of Aspergillus spp. was evaluated with an interlaboratory quality control programme panel and human bronchoalveolar lavage fluid (BALF) samples. With the quality...
8.
Bustin S, Huggett J
Biomol Detect Quantif
. 2017 Dec;
14:19-28.
PMID: 29201647
Primers are arguably the single most critical components of any PCR assay, as their properties control the exquisite specificity and sensitivity that make this method uniquely powerful. Consequently, poor design...
9.
Bustin S, Nolan T
Eur J Clin Invest
. 2017 Aug;
47(10):756-774.
PMID: 28796277
Poorly executed and inadequately reported molecular measurement methods are amongst the causes underlying the lack of reproducibility of much biomedical research. Although several high impact factor journals have acknowledged their...
10.
Bustin S
Biomol Detect Quantif
. 2017 Jul;
12:7-9.
PMID: 28702367
Attendance at this year's European Calcified Tissue Society's (ECTS) Congress reveals that the methods used to obtain qPCR results continue to be significantly flawed and that and their reporting remain...