Shouichi Sakakihara
Overview
Explore the profile of Shouichi Sakakihara including associated specialties, affiliations and a list of published articles.
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Articles
14
Citations
316
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0
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Recent Articles
1.
Yamaguchi S, Takagi R, Hosogane T, Ohashi Y, Sakai Y, Sakakihara S, et al.
ACS Appl Bio Mater
. 2022 Jan;
3(9):5887-5895.
PMID: 35021817
Single cell arrays provide an accurate classification of analyte cells through an image-based analysis of cellular phenotypes. Light-guided cell retrieval from a single cell array is a promising approach for...
2.
Iino R, Sakakihara S, Matsumoto Y, Nishino K
Methods Mol Biol
. 2017 Nov;
1700:331-341.
PMID: 29177839
Large-scale femtoliter droplet array as a platform for single cell efflux assay of bacteria is described. Device microfabrication, femtoliter droplet array formation and concomitant enclosure of single bacterial cells, fluorescence-based...
3.
Matsumoto Y, Sakakihara S, Grushnikov A, Kikuchi K, Noji H, Yamaguchi A, et al.
PLoS One
. 2016 Feb;
11(2):e0148797.
PMID: 26872134
The recent global increase in the prevalence of antibiotic-resistant bacteria and lack of development of new therapeutic agents emphasize the importance of selecting appropriate antimicrobials for the treatment of infections....
4.
Iino R, Sakakihara S, Matsumoto Y, Nishino K
Methods Mol Biol
. 2015 Oct;
1333:101-9.
PMID: 26468103
A directly accessible femtoliter droplet array as a platform for single-cell detection and collection of persister bacteria is described. Device microfabrication, femtoliter droplet array formation and concomitant enclosure of single...
5.
Enoki S, Iino R, Morone N, Kaihatsu K, Sakakihara S, Kato N, et al.
PLoS One
. 2012 Nov;
7(11):e49208.
PMID: 23166613
Here we report label-free optical imaging of single particles of the influenza virus attached on a glass surface with a simple objective-type total internal reflection dark-field microscopy (TIRDFM). The capability...
6.
Kim S, Iwai S, Araki S, Sakakihara S, Iino R, Noji H
Lab Chip
. 2012 Sep;
12(23):4986-91.
PMID: 22961607
We present a novel device employing one million femtoliter droplets immobilized on a substrate for the quantitative detection of extremely low concentrations of biomolecules in a sample. Surface-modified polystyrene beads...
7.
Iino R, Hayama K, Amezawa H, Sakakihara S, Kim S, Matsumono Y, et al.
Lab Chip
. 2012 Jul;
12(20):3923-9.
PMID: 22814576
Active efflux of drugs, such as antibiotics, from a cell is one of the major mechanisms that cause multi-drug resistance in bacteria. Here we report a method to assess drug...
8.
Watanabe R, Okuno D, Sakakihara S, Shimabukuro K, Iino R, Yoshida M, et al.
Nat Chem Biol
. 2011 Nov;
8(1):86-92.
PMID: 22101603
The conformational fluctuation of enzymes has a crucial role in reaction acceleration. However, the contribution to catalysis enhancement of individual substates with conformations far from the average conformation remains unclear....
9.
Matsumoto Y, Hayama K, Sakakihara S, Nishino K, Noji H, Iino R, et al.
PLoS One
. 2011 May;
6(4):e18547.
PMID: 21533264
Fluorescein-di-β-D-galactopyranoside (FDG), a fluorogenic compound, is hydrolyzed by β-galactosidase in the cytoplasm of Escherichia coli to produce a fluorescent dye, fluorescein. We found that both FDG and fluorescein were substrates...
10.
Sakakihara S, Araki S, Iino R, Noji H
Lab Chip
. 2010 Oct;
10(24):3355-62.
PMID: 21031171
The enzyme assay in a femtoliter chamber array is a simple and efficient method for concentrating the reaction product; it greatly improves the detection sensitivity down to the single-molecule level....