Richard B Hitchman
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Explore the profile of Richard B Hitchman including associated specialties, affiliations and a list of published articles.
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12
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184
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Recent Articles
1.
Black C, Barker J, Hitchman R, Kwong H, Festenstein S, Acton T
Methods Mol Biol
. 2017 May;
1586:359-371.
PMID: 28470618
We have developed a standardized and efficient workflow for high-throughput (HT) protein expression in E. coli and parallel purification which can be tailored to the downstream application of the target...
2.
Hitchman E, Hitchman R, King L
Hum Gene Ther
. 2017 Jan;
28(9):747-756.
PMID: 28042948
Ischemia-reperfusion (I/R) injury remains the primary contributor to delayed graft function in kidney transplantation. The beneficial application of manganese superoxide dismutase (sod), delivered by a BacMam vector, against renal I/R...
3.
Hitchman R, Possee R, King L
Methods Mol Biol
. 2011 Dec;
824:609-27.
PMID: 22160923
Historically, it has been proved difficult to adapt the traditional baculovirus expression systems to an automated platform because of the complexity of the processes involved. One of the major bottlenecks...
4.
Hitchman R, Locanto E, Possee R, King L
Methods
. 2011 Sep;
55(1):52-7.
PMID: 21945427
Baculoviruses have a unique bi-phasic life cycle and powerful promoters, which greatly facilitates their use for recombinant protein expression in insect cells. We have developed an expression system that utilizes...
5.
Hitchman R, Murguia-Meca F, Locanto E, Danquah J, King L
J Invertebr Pathol
. 2011 Jul;
107 Suppl:S49-58.
PMID: 21784231
The baculovirus Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is able to transduce a wide range of mammalian cells and shows preferential uptake in some, particularly liver and kidney cells. This suggests...
6.
Murguia-Meca F, Plata-Munoz J, Hitchman R, Danquah J, Hughes D, Friend P, et al.
Transpl Int
. 2011 Jun;
24(8):820-8.
PMID: 21623952
Concerns over the safety of conventional viral vectors have limited the translation of gene transfer from an exciting experimental procedure to a successful clinical therapy in transplantation. Baculoviruses are insect...
7.
Hitchman R, Possee R, Siaterli E, Richards K, Clayton A, Bird L, et al.
Biotechnol Appl Biochem
. 2010 May;
56(3):85-93.
PMID: 20441568
Secretory and membrane-bound proteins are generally produced in lower amounts in insect cells compared with cytoplasmic and nuclear proteins. There may be many reasons for this, including degradation of recombinant...
8.
Hitchman R, Possee R, Crombie A, Chambers A, Ho K, Siaterli E, et al.
Cell Biol Toxicol
. 2009 Aug;
26(1):57-68.
PMID: 19655260
Generating large amounts of recombinant protein in transgenic animals is often challenging and has a number of drawbacks compared to cell culture systems. The baculovirus expression vector system (BEVS) uses...
9.
Hitchman R, Possee R, King L
Recent Pat Biotechnol
. 2009 Jan;
3(1):46-54.
PMID: 19149722
Baculoviruses are lethal pathogens of insects, predominantly of the order Lepidoptera. These viruses have a bi-phasic life cycle, which greatly facilitates their use for biotechnological applications. They were exploited initially...
10.
Possee R, Hitchman R, Richards K, Mann S, Siaterli E, Nixon C, et al.
Biotechnol Bioeng
. 2008 Sep;
101(6):1115-22.
PMID: 18781697
The baculovirus expression system is one of the most popular methods used for the production of recombinant proteins but has several complex steps which have proved inherently difficult to adapt...