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» Authors » Rafal Tomecki

Rafal Tomecki

Explore the profile of Rafal Tomecki including associated specialties, affiliations and a list of published articles. Areas
Snapshot
Articles 39
Citations 1394
Followers 0
Related Specialties
Biochemistry
Molecular Biology
Biology
Top 10 Co-Authors
Andrzej Dziembowski
Karolina Drazkowska
Agnieszka Tudek
Aleksander Chlebowski
Kamil Kobylecki
Torben Heick Jensen
Piotr P Stepien
Dominik Cysewski
Anna Labno
Katarzyna Kowalska
Published In
Nucleic Acids Res
EMBO J
RNA Biol
RNA
Chembiochem
Affiliations
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Recent Articles
1.
Purification of Enzymatically Active Xrn1 for Removal of Non-capped mRNAs from In Vitro Transcription Reactions and Evaluation of mRNA Decapping Status In Vivo
Drazkowska K, Tomecki R, Tudek A
Methods Mol Biol . 2024 Nov; 2863:81-105. PMID: 39535706
The cap is a 7-methylguanosine attached to the first messenger RNA (mRNA) nucleotide with a 5'-5' triphosphate bridge. This conserved eukaryotic modification confers stability to the transcripts and is essential...
2.
Modeling of mRNA deadenylation rates reveal a complex relationship between mRNA deadenylation and decay
Czarnocka-Cieciura A, Poznanski J, Turtola M, Tomecki R, Krawczyk P, Mroczek S, et al.
EMBO J . 2024 Oct; 43(24):6525-6554. PMID: 39394354
Complete cytoplasmic polyadenosine tail (polyA-tail) deadenylation is thought to be essential for initiating mRNA decapping and subsequent degradation. To investigate this prevalent model, we conducted direct RNA sequencing of S....
3.
Expanding the Available RNA Labeling Toolbox With CutA Nucleotidyltransferase for Efficient Transcript Labeling with Purine and Pyrimidine Nucleotide Analogs
Tomecki R, Drazkowska K, Madaj R, Mamot A, Dunin-Horkawicz S, Sikorski P
Chembiochem . 2024 May; 25(15):e202400202. PMID: 38818670
RNA labeling is an invaluable tool for investigation of the function and localization of nucleic acids. Labels are commonly incorporated into 3' end of RNA and the primary enzyme used...
4.
The strain-dependent cytostatic activity of Lactococcus lactis on CRC cell lines is mediated through the release of arginine deiminase
Jastrzab R, Tomecki R, Jurkiewicz A, Graczyk D, Szczepankowska A, Mytych J, et al.
Microb Cell Fact . 2024 Mar; 23(1):82. PMID: 38481270
Background: Colorectal cancer (CRC) is one of the most commonly diagnosed cancers, posing a serious public health challenge that necessitates the development of new therapeutics, therapies, and prevention methods. Among...
5.
SKI complex: A multifaceted cytoplasmic RNA exosome cofactor in mRNA metabolism with links to disease, developmental processes, and antiviral responses
Tomecki R, Drazkowska K, Kobylecki K, Tudek A
Wiley Interdiscip Rev RNA . 2023 Jun; 14(6):e1795. PMID: 37384835
RNA stability and quality control are integral parts of gene expression regulation. A key factor shaping eukaryotic transcriptomes, mainly via 3'-5' exoribonucleolytic trimming or degradation of diverse transcripts in nuclear...
6.
A cap 0-dependent mRNA capture method to analyze the yeast transcriptome
Nowacka M, Latoch P, Izert M, Karolak N, Tomecki R, Koper M, et al.
Nucleic Acids Res . 2022 Oct; 50(22):e132. PMID: 36259646
Analysis of the protein coding transcriptome by the RNA sequencing requires either enrichment of the desired fraction of coding transcripts or depletion of the abundant non-coding fraction consisting mainly of...
7.
The RNA-Binding Landscape of HAX1 Protein Indicates Its Involvement in Translation and Ribosome Assembly
Balcerak A, Macech-Klicka E, Wakula M, Tomecki R, Goryca K, Rydzanicz M, et al.
Cells . 2022 Oct; 11(19). PMID: 36230905
HAX1 is a human protein with no known homologues or structural domains. Mutations in the gene cause severe congenital neutropenia through mechanisms that are poorly understood. Previous studies reported the...
8.
2'-O-Methylation of the second transcribed nucleotide within the mRNA 5' cap impacts the protein production level in a cell-specific manner and contributes to RNA immune evasion
Drazkowska K, Tomecki R, Warminski M, Baran N, Cysewski D, Depaix A, et al.
Nucleic Acids Res . 2022 Aug; 50(16):9051-9071. PMID: 36018811
In mammals, m7G-adjacent nucleotides undergo extensive modifications. Ribose of the first or first and second transcribed nucleotides can be subjected to 2'-O-methylation to form cap1 or cap2, respectively. When the...
9.
Reproducible and efficient new method of RNA 3'-end labelling by CutA nucleotidyltransferase-mediated CC-tailing
Tomecki R, Kobylecki K, Drazkowska K, Hyjek-Skladanowska M, Dziembowski A
RNA Biol . 2021 Nov; 18(sup2):623-639. PMID: 34766865
Despite the development of non-radioactive DNA/RNA labelling methods, radiolabelled nucleic acids are commonly used in studies focused on the determination of RNA fate. Nucleic acid fragments with radioactive nucleotide analoguesincorporated...
10.
Global view on the metabolism of RNA poly(A) tails in yeast Saccharomyces cerevisiae
Tudek A, Krawczyk P, Mroczek S, Tomecki R, Turtola M, Matylla-Kulinska K, et al.
Nat Commun . 2021 Aug; 12(1):4951. PMID: 34400637
The polyadenosine tail (poly[A]-tail) is a universal modification of eukaryotic messenger RNAs (mRNAs) and non-coding RNAs (ncRNAs). In budding yeast, Pap1-synthesized mRNA poly(A) tails enhance export and translation, whereas Trf4/5-mediated...