Rafal Tomecki

Overview

Explore the profile of Rafal Tomecki including associated specialties, affiliations and a list of published articles.

Snapshot

Articles 39

Citations 1394

Followers 0

Related Specialties

Biochemistry

Molecular Biology

Biology

Top 10 Co-Authors

Andrzej Dziembowski

Karolina Drazkowska

Agnieszka Tudek

Aleksander Chlebowski

Kamil Kobylecki

Torben Heick Jensen

Piotr P Stepien

Dominik Cysewski

Anna Labno

Katarzyna Kowalska

Published In

Nucleic Acids Res

EMBO J

RNA Biol

RNA

Chembiochem

Affiliations

Soon will be listed here.

Recent Articles

11.

An integrative approach uncovers transcriptome-wide determinants of mRNA stability regulation in Saccharomyces cerevisiae

Tomecki R, Drazkowska K

FEBS J . 2021 Feb; 288(11):3418-3423. PMID: 33590687

mRNA degradation rate is one of the key stages of gene expression regulation in eukaryotic cells. To date, intertwined processes of post-transcriptional control have been widely investigated, but focused rather...

12.

Structure and mechanism of CutA, RNA nucleotidyl transferase with an unusual preference for cytosine

Malik D, Kobylecki K, Krawczyk P, Poznanski J, Jakielaszek A, Napiorkowska A, et al.

Nucleic Acids Res . 2020 Aug; 48(16):9387-9405. PMID: 32785623

Template-independent terminal ribonucleotide transferases (TENTs) catalyze the addition of nucleotide monophosphates to the 3'-end of RNA molecules regulating their fate. TENTs include poly(U) polymerases (PUPs) with a subgroup of 3'...

13.

Elimination of 01/A'-A0 pre-rRNA processing by-product in human cells involves cooperative action of two nuclear exosome-associated nucleases: RRP6 and DIS3

Kobylecki K, Drazkowska K, Kulinski T, Dziembowski A, Tomecki R

RNA . 2018 Sep; 24(12):1677-1692. PMID: 30266864

Pre-rRNA processing generates mature 18S, 5.8S, and 28S/25S rRNAs through multistage removal of surrounding 5'-ETS/3'-ETS and intervening ITS1/ITS2 segments. Endonucleolytic activities release by-products, which need to be eliminated. Here, we...

14.

Versatile approach for functional analysis of human proteins and efficient stable cell line generation using FLP-mediated recombination system

Szczesny R, Kowalska K, Klosowska-Kosicka K, Chlebowski A, Owczarek E, Warkocki Z, et al.

PLoS One . 2018 Mar; 13(3):e0194887. PMID: 29590189

Deciphering a function of a given protein requires investigating various biological aspects. Usually, the protein of interest is expressed with a fusion tag that aids or allows subsequent analyses. Additionally,...

15.

Biochemical and structural bioinformatics studies of fungal CutA nucleotidyltransferases explain their unusual specificity toward CTP and increased tendency for cytidine incorporation at the 3'-terminal positions of synthesized tails

Kobylecki K, Kuchta K, Dziembowski A, Ginalski K, Tomecki R

RNA . 2017 Sep; 23(12):1902-1926. PMID: 28947555

Noncanonical RNA nucleotidyltransferases (NTases), including poly(A), poly(U) polymerases (PAPs/PUPs), and C/U-adding enzymes, modify 3'-ends of different transcripts affecting their functionality and stability. They contain PAP/OAS1 substrate-binding domain (SBD) with inserted...

16.

Comparison of preribosomal RNA processing pathways in yeast, plant and human cells - focus on coordinated action of endo- and exoribonucleases

Tomecki R, Sikorski P, Zakrzewska-Placzek M

FEBS Lett . 2017 May; 591(13):1801-1850. PMID: 28524231

Proper regulation of ribosome biosynthesis is mandatory for cellular adaptation, growth and proliferation. Ribosome biogenesis is the most energetically demanding cellular process, which requires tight control. Abnormalities in ribosome production...

17.

A short splicing isoform of HBS1L links the cytoplasmic exosome and SKI complexes in humans

Kalisiak K, Kulinski T, Tomecki R, Cysewski D, Pietras Z, Chlebowski A, et al.

Nucleic Acids Res . 2017 Feb; 45(4):2068-2080. PMID: 28204585

The exosome complex is a major eukaryotic exoribonuclease that requires the SKI complex for its activity in the cytoplasm. In yeast, the Ski7 protein links both complexes, whereas a functional...

18.

Cytoplasmic RNA decay pathways - Enzymes and mechanisms

Labno A, Tomecki R, Dziembowski A

Biochim Biophys Acta . 2016 Oct; 1863(12):3125-3147. PMID: 27713097

RNA decay plays a crucial role in post-transcriptional regulation of gene expression. Work conducted over the last decades has defined the major mRNA decay pathways, as well as enzymes and...

19.

Perlman syndrome nuclease DIS3L2 controls cytoplasmic non-coding RNAs and provides surveillance pathway for maturing snRNAs

Labno A, Warkocki Z, Kulinski T, Krawczyk P, Bijata K, Tomecki R, et al.

Nucleic Acids Res . 2016 Jul; 44(21):10437-10453. PMID: 27431325

The exosome-independent exoribonuclease DIS3L2 is mutated in Perlman syndrome. Here, we used extensive global transcriptomic and targeted biochemical analyses to identify novel DIS3L2 substrates in human cells. We show that...

20.

DIS3 shapes the RNA polymerase II transcriptome in humans by degrading a variety of unwanted transcripts

Szczepinska T, Kalisiak K, Tomecki R, Labno A, Borowski L, Kulinski T, et al.

Genome Res . 2015 Aug; 25(11):1622-33. PMID: 26294688

Human DIS3, the nuclear catalytic subunit of the exosome complex, contains exonucleolytic and endonucleolytic active domains. To identify DIS3 targets genome-wide, we combined comprehensive transcriptomic analyses of engineered HEK293 cells...