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» Authors » Olga Musharova

Olga Musharova

Explore the profile of Olga Musharova including associated specialties, affiliations and a list of published articles. Areas
Snapshot
Articles 13
Citations 231
Followers 0
Related Specialties
Biochemistry
Science
Biology
Top 10 Co-Authors
Konstantin Severinov
Ekaterina Semenova
Ekaterina Savitskaya
Polina Selkova
Anatolii Arseniev
Iana Fedorova
Georgii Pobegalov
Tatyana Artamonova
Tatyana Zyubko
Mikhail Khodorkovskii
Published In
Nucleic Acids Res
Proc Natl Acad Sci U S A
Mol Microbiol
Sci Adv
RNA Biol
Affiliations
Soon will be listed here.
Recent Articles
1.
RecA-dependent or independent recombination of plasmid DNA generates a conflict with the host EcoKI immunity by launching restriction alleviation
Skutel M, Yanovskaya D, Demkina A, Shenfeld A, Musharova O, Severinov K, et al.
Nucleic Acids Res . 2024 Apr; 52(9):5195-5208. PMID: 38567730
Bacterial defence systems are tightly regulated to avoid autoimmunity. In Type I restriction-modification (R-M) systems, a specific mechanism called restriction alleviation (RA) controls the activity of the restriction module. In...
2.
Host nucleases generate prespacers for primed adaptation in the type I-E CRISPR-Cas system
Shiriaeva A, Kuznedelov K, Fedorov I, Musharova O, Khvostikov T, Tsoy Y, et al.
Sci Adv . 2022 Nov; 8(47):eabn8650. PMID: 36427302
CRISPR-Cas systems provide prokaryotes with adaptive immunity against foreign nucleic acids. In , immunity is acquired upon integration of 33-bp spacers into CRISPR arrays. DNA targets complementary to spacers get...
3.
Interaction between transcribing RNA polymerase and topoisomerase I prevents R-loop formation in E. coli
Sutormin D, Galivondzhyan A, Musharova O, Travin D, Rusanova A, Obraztsova K, et al.
Nat Commun . 2022 Aug; 13(1):4524. PMID: 35927234
Bacterial topoisomerase I (TopoI) removes excessive negative supercoiling and is thought to relax DNA molecules during transcription, replication and other processes. Using ChIP-Seq, we show that TopoI of Escherichia coli...
4.
Prespacers formed during primed adaptation associate with the Cas1-Cas2 adaptation complex and the Cas3 interference nuclease-helicase
Musharova O, Medvedeva S, Klimuk E, Guzman N, Titova D, Zgoda V, et al.
Proc Natl Acad Sci U S A . 2021 May; 118(22). PMID: 34035168
For Type I CRISPR-Cas systems, a mode of CRISPR adaptation named priming has been described. Priming allows specific and highly efficient acquisition of new spacers from DNA recognized (primed) by...
5.
PpCas9 from Pasteurella pneumotropica - a compact Type II-C Cas9 ortholog active in human cells
Fedorova I, Vasileva A, Selkova P, Abramova M, Arseniev A, Pobegalov G, et al.
Nucleic Acids Res . 2020 Nov; 48(21):12297-12309. PMID: 33152077
CRISPR-Cas defense systems opened up the field of genome editing due to the ease with which effector Cas nucleases can be programmed with guide RNAs to access desirable genomic sites....
6.
Position of Deltaproteobacteria Cas12e nuclease cleavage sites depends on spacer length of guide RNA
Selkova P, Vasileva A, Pobegalov G, Musharova O, Arseniev A, Kazalov M, et al.
RNA Biol . 2020 Jun; 17(10):1472-1479. PMID: 32564655
Cas12e proteins (formerly CasX) form a distinct subtype of Class II type V CRISPR-Cas effectors. Recently, it was shown that DpbCas12e from and PlmCas12e from can introduce programmable double-stranded breaks...
7.
DNA targeting by Clostridium cellulolyticum CRISPR-Cas9 Type II-C system
Fedorova I, Arseniev A, Selkova P, Pobegalov G, Goryanin I, Vasileva A, et al.
Nucleic Acids Res . 2020 Jan; 48(4):2026-2034. PMID: 31943070
Type II CRISPR-Cas9 RNA-guided nucleases are widely used for genome engineering. Type II-A SpCas9 protein from Streptococcus pyogenes is the most investigated and highly used enzyme of its class. Nevertheless,...
8.
Systematic analysis of Type I-E Escherichia coli CRISPR-Cas PAM sequences ability to promote interference and primed adaptation
Musharova O, Sitnik V, Vlot M, Savitskaya E, Datsenko K, Krivoy A, et al.
Mol Microbiol . 2019 Mar; 111(6):1558-1570. PMID: 30875129
CRISPR interference occurs when a protospacer recognized by the CRISPR RNA is destroyed by Cas effectors. In Type I CRISPR-Cas systems, protospacer recognition can lead to «primed adaptation» - acquisition...
9.
Avoidance of Trinucleotide Corresponding to Consensus Protospacer Adjacent Motif Controls the Efficiency of Prespacer Selection during Primed Adaptation
Musharova O, Vyhovskyi D, Medvedeva S, Guzina J, Zhitnyuk Y, Djordjevic M, et al.
mBio . 2018 Dec; 9(6). PMID: 30514784
CRISPR DNA arrays of unique spacers separated by identical repeats ensure prokaryotic immunity through specific targeting of foreign nucleic acids complementary to spacers. New spacers are acquired into a CRISPR...
10.
Primed CRISPR adaptation in Escherichia coli cells does not depend on conformational changes in the Cascade effector complex detected in Vitro
Krivoy A, Rutkauskas M, Kuznedelov K, Musharova O, Rouillon C, Severinov K, et al.
Nucleic Acids Res . 2018 Mar; 46(8):4087-4098. PMID: 29596641
In type I CRISPR-Cas systems, primed adaptation of new spacers into CRISPR arrays occurs when the effector Cascade-crRNA complex recognizes imperfectly matched targets that are not subject to efficient CRISPR...