N T Ktistakis
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Explore the profile of N T Ktistakis including associated specialties, affiliations and a list of published articles.
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25
Citations
814
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Recent Articles
1.
Walker S, Karanasios E, Ktistakis N
Methods Enzymol
. 2017 Mar;
587:1-20.
PMID: 28253951
Autophagy is a highly dynamic intracellular process involving interactions between protein complexes and membranes. Direct observation of these components in living cells provides information on how they interact and when...
2.
Pyne S, Long J, Ktistakis N, Pyne N
Biochem Soc Trans
. 2005 Oct;
33(Pt 6):1370-4.
PMID: 16246121
Mammalian LPPs (lipid phosphate phosphatases) are integral membrane proteins that belong to a superfamily of lipid phosphatases/phosphotransferases. They have broad substrate specificity in vitro, dephosphorylating PA (phosphatidic acid), S1P (sphingosine...
3.
Loewen C, Gaspar M, Jesch S, Delon C, Ktistakis N, Henry S, et al.
Science
. 2004 Jun;
304(5677):1644-7.
PMID: 15192221
Cells regulate the biophysical properties of their membranes by coordinated synthesis of different classes of lipids. Here, we identified a highly dynamic feedback mechanism by which the budding yeast Saccharomyces...
4.
Ktistakis N, Delon C, Manifava M, Wood E, Ganley I, Sugars J
Biochem Soc Trans
. 2003 Jan;
31(Pt 1):94-7.
PMID: 12546662
Phospholipase D (PLD) hydrolyses phosphatidylcholine into phosphatidic acid (PA) and choline. Our work aims to understand the properties of PLD1, and to identify downstream targets of PA. In one set...
5.
Lucocq J, Manifava M, Bi K, Roth M, Ktistakis N
Eur J Cell Biol
. 2001 Sep;
80(8):508-20.
PMID: 11561902
We have examined the localisation of overexpressed phospholipase D1 (PLD1) using antibodies against its amino- and carboxyl-terminal domains. PLD1 overexpressed in COS-7 cells showed variable distribution by immunofluorescence but was...
6.
Biological properties and measurement of phospholipase D activation by ADP-ribosylation factor (ARF)
Cockcroft S, Bi K, Ktistakis N, Roth M
Methods Enzymol
. 2001 Feb;
329:355-72.
PMID: 11210555
No abstract available.
7.
Ganley I, Walker S, Manifava M, Li D, Brown H, Ktistakis N
Biochem J
. 2001 Feb;
354(Pt 2):369-78.
PMID: 11171116
Phospholipase D (PLD)1 was phosphorylated in vivo and by an associated kinase in vitro following immunoprecipitation. Both phosphorylation events were greatly reduced in a catalytically inactive point mutant in which...
8.
Manifava M, Thuring J, Lim Z, PACKMAN L, Holmes A, Ktistakis N
J Biol Chem
. 2001 Jan;
276(12):8987-94.
PMID: 11124268
Phosphatidic acid (PA) is an important bioactive lipid, but its molecular targets remain unknown. To identify such targets, we have synthesized and coupled PA to an agarose-based matrix, Affi-Gel 10....
9.
Ktistakis N, Manifava M, Sugars J, Bi K, Roth M
Biochem Soc Trans
. 2000 Aug;
27(4):634-7.
PMID: 10917656
No abstract available.
10.
Sugars J, Cellek S, Manifava M, Coadwell J, Ktistakis N
J Biol Chem
. 1999 Oct;
274(42):30023-7.
PMID: 10514487
We have reported previously that phospholipase D1 (PLD1) is labeled specifically with [(3)H]palmitate following transient expression and immunoprecipitation and that this modification appeared important both for membrane localization and catalytic...