M Manifava
Overview
Explore the profile of M Manifava including associated specialties, affiliations and a list of published articles.
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Articles
12
Citations
244
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Recent Articles
1.
Ktistakis N, Delon C, Manifava M, Wood E, Ganley I, Sugars J
Biochem Soc Trans
. 2003 Jan;
31(Pt 1):94-7.
PMID: 12546662
Phospholipase D (PLD) hydrolyses phosphatidylcholine into phosphatidic acid (PA) and choline. Our work aims to understand the properties of PLD1, and to identify downstream targets of PA. In one set...
2.
Krugmann S, Anderson K, Ridley S, Risso N, McGregor A, Coadwell J, et al.
Mol Cell
. 2002 Jan;
9(1):95-108.
PMID: 11804589
We show that matrices carrying the tethered homologs of natural phosphoinositides can be used to capture and display multiple phosphoinositide binding proteins in cell and tissue extracts. We present the...
3.
Ridley S, Ktistakis N, Davidson K, Anderson K, Manifava M, Ellson C, et al.
J Cell Sci
. 2001 Dec;
114(Pt 22):3991-4000.
PMID: 11739631
FENS-1 and DFCP1 are recently discovered proteins containing one or two FYVE-domains respectively. We show that the FYVE domains in these proteins can bind PtdIns3P in vitro with high specificity...
4.
Lucocq J, Manifava M, Bi K, Roth M, Ktistakis N
Eur J Cell Biol
. 2001 Sep;
80(8):508-20.
PMID: 11561902
We have examined the localisation of overexpressed phospholipase D1 (PLD1) using antibodies against its amino- and carboxyl-terminal domains. PLD1 overexpressed in COS-7 cells showed variable distribution by immunofluorescence but was...
5.
Ganley I, Walker S, Manifava M, Li D, Brown H, Ktistakis N
Biochem J
. 2001 Feb;
354(Pt 2):369-78.
PMID: 11171116
Phospholipase D (PLD)1 was phosphorylated in vivo and by an associated kinase in vitro following immunoprecipitation. Both phosphorylation events were greatly reduced in a catalytically inactive point mutant in which...
6.
Manifava M, Thuring J, Lim Z, PACKMAN L, Holmes A, Ktistakis N
J Biol Chem
. 2001 Jan;
276(12):8987-94.
PMID: 11124268
Phosphatidic acid (PA) is an important bioactive lipid, but its molecular targets remain unknown. To identify such targets, we have synthesized and coupled PA to an agarose-based matrix, Affi-Gel 10....
7.
Ktistakis N, Manifava M, Sugars J, Bi K, Roth M
Biochem Soc Trans
. 2000 Aug;
27(4):634-7.
PMID: 10917656
No abstract available.
8.
Sugars J, Cellek S, Manifava M, Coadwell J, Ktistakis N
J Biol Chem
. 1999 Oct;
274(42):30023-7.
PMID: 10514487
We have reported previously that phospholipase D1 (PLD1) is labeled specifically with [(3)H]palmitate following transient expression and immunoprecipitation and that this modification appeared important both for membrane localization and catalytic...
9.
Manifava M, Sugars J, Ktistakis N
J Biol Chem
. 1999 Jan;
274(2):1072-7.
PMID: 9873053
Phospholipase D1 (PLD1) was covalently labeled with 3H when expressed transiently in COS cells and immunoprecipitated following labeling of the cells with [3H]palmitate. Labeling of PLD1 was abolished by treatment...
10.
Goulielmos G, Manifava M, Moschonas N
Hum Mol Genet
. 1993 Dec;
2(12):2202.
PMID: 8111403
No abstract available.