Koen J T Venken
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Explore the profile of Koen J T Venken including associated specialties, affiliations and a list of published articles.
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43
Citations
3549
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Recent Articles
1.
Venken K, Matinyan N, Gonzalez Y, Dierick H
Curr Protoc
. 2023 Feb;
3(2):e675.
PMID: 36757632
Transgenes with genomic DNA fragments that encompass genes of interest are the gold standard for complementing null alleles in rescue experiments in the fruit fly Drosophila melanogaster. Of particular interest...
2.
Venken K, Matinyan N, Gonzalez Y, Sarrion-Perdigones A, Dierick H
Curr Protoc
. 2023 Feb;
3(2):e653.
PMID: 36757602
We recently described a drug-based selectable and counterselectable genetic platform for the animal model system Drosophila melanogaster, consisting of four resistance and two sensitivity markers that allow direct selection for,...
3.
Venken K, Matinyan N, Gonzalez Y, Dierick H
Curr Protoc
. 2023 Feb;
3(2):e652.
PMID: 36757287
We recently described a set of four selectable and two counterselectable markers that provide resistance and sensitivity, respectively, against their corresponding drugs using the model organism Drosophila melanogaster. The four...
4.
Sarrion-Perdigones A, Gonzalez Y, Chang L, Gallego-Flores T, Young D, Venken K
Methods Mol Biol
. 2022 Jul;
2524:433-456.
PMID: 35821491
We recently expanded the commonly used dual luciferase assaying method toward multiplex hextuple luciferase assaying, allowing monitoring the activity of five experimental pathways against one control at the same time....
5.
Sarrion-Perdigones A, Gonzalez Y, Venken K
Methods Mol Biol
. 2022 Jul;
2524:409-432.
PMID: 35821490
Multiplex hextuple luciferase assaying allows monitoring the activity of five experimental pathways against one control at the same time. To perform multiplex hextuple luciferase assaying, six orthogonal luciferase reporter units...
6.
Chmielowiec J, Szlachcic W, Yang D, Scavuzzo M, Wamble K, Sarrion-Perdigones A, et al.
Nat Commun
. 2022 Apr;
13(1):1952.
PMID: 35414140
In vitro derivation of pancreatic β-cells from human pluripotent stem cells holds promise as diabetes treatment. Despite recent progress, efforts to generate physiologically competent β-cells are still hindered by incomplete...
7.
Matinyan N, Gonzalez Y, Dierick H, Venken K
STAR Protoc
. 2021 Sep;
2(3):100783.
PMID: 34585147
We recently integrated into fly genetics a set of four selection and two counterselection markers and their corresponding drugs that can be used individually or in combination. These markers eliminate...
8.
Matinyan N, Karkhanis M, Gonzalez Y, Jain A, Saltzman A, Malovannaya A, et al.
Cell Rep
. 2021 Sep;
36(11):109700.
PMID: 34525356
The power of Drosophila melanogaster as a model system relies on tractable germline genetic manipulations. Despite Drosophila's expansive genetics toolbox, such manipulations are still accomplished one change at a time...
9.
Wang J, Liu H, Bertrand R, Sarrion-Perdigones A, Gonzalez Y, Venken K, et al.
Genet Med
. 2020 Sep;
23(1):59-68.
PMID: 32884132
Purpose: To achieve the ultimate goal of personalized treatment of patients, accurate molecular diagnosis and precise interpretation of the impact of genetic variants on gene function is essential. With sequencing...
10.
Sarrion-Perdigones A, Chang L, Gonzalez Y, Gallego-Flores T, Young D, Venken K
Curr Protoc Mol Biol
. 2020 Jun;
131(1):e122.
PMID: 32539239
Multiplex experimentation that can assay multiple cellular signaling pathways in the same cells requires orthogonal genetically encoded reporters that report over large dynamic ranges. Luciferases are cost-effective, versatile candidates whose...