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Kiyoshi Yasukawa

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Articles 116
Citations 320
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Recent Articles
1.
Yasuda M, Pham N, Hirakawa Y, Momma K, Takita T, Tsuboi M, et al.
Biosci Biotechnol Biochem . 2025 Feb; PMID: 39900469
Bacteriophage T4 gene 32 protein (gp32) preferentially binds to single-stranded DNA (ssDNA) to facilitate DNA replication but shows weak binding to double-stranded DNA (dsDNA). Polyclonal and monoclonal antibodies against gp32...
2.
Takita T, Wada M, Yamagata M, Kamata S, Mizutani K, Yogo Y, et al.
Biochemistry . 2025 Jan; 64(2):468-478. PMID: 39752145
CYP105A1 exhibits monooxygenase activity to a wide variety of structurally different substrates with regio- and stereospecificity, making its application range broad. Our previous studies have shown that CYP105A1 wild type...
3.
Nishimoto S, Debarbat A, Ikeda Y, Arikawa E, Odagaki Y, Yano H, et al.
J Agric Food Chem . 2024 Dec; 73(1):617-624. PMID: 39714016
In mammals, intestinal α-glucosidase exists as a maltase-glucoamylase complex (MGAM) and a sucrase-isomaltase complex (SI). In this study, we transiently expressed human MGAM and SI in human embryonic kidney 293...
4.
Martinez Del Rio J, Frutos-Beltran E, Sebastian-Martin A, Lasala F, Yasukawa K, Delgado R, et al.
J Mol Biol . 2024 Oct; 436(22):168815. PMID: 39384034
Nucleotide incorporation and lacZ-based forward mutation assays have been widely used to determine the accuracy of reverse transcriptases (RTs) in RNA-dependent DNA polymerization reactions. However, they involve quite complex and...
5.
Odagaki Y, Murakami Y, Takita T, Mizutani K, Mikami B, Fujiwara S, et al.
Biochem Biophys Res Commun . 2024 Sep; 733:150728. PMID: 39321488
Agmatine, a natural polyamine also known as 4-aminobutyl-guanidine, is biosynthesized from arginine by decarboxylation. Aspergillus oryzae contains high amounts of agmatine, suggesting highly active arginine decarboxylase (ADC) in this organism....
6.
Wu H, Fujisawa Y, Tozuka Z, Fomenkov A, Nakura Y, Kajiyama S, et al.
Enzyme Microb Technol . 2024 Jul; 180:110471. PMID: 38959818
Here, we report a novel endonuclease and N-adenine DNA methyltransferase (mA methyltransferase) in the Ureaplasma parvum SV3F4 strain. Our previous study found that the SV3F4 strain carries 17 unique genes,...
7.
Yoshida Y, Niimi Y, Fushihara D, Katakura H, Fukui R, Murase H, et al.
Bioorg Med Chem . 2024 Jul; 110:117813. PMID: 38954919
Nucleoside reverse transcriptase inhibitors (NRTIs) have been extensively studied as drugs targeting HIV RT. However, the practice or use of approved NRTIs lacking the 3'-hydroxy group often promotes frequent HIV...
8.
Juma K, Murakami Y, Morimoto K, Takita T, Kojima K, Suzuki K, et al.
J Biosci Bioeng . 2024 May; 138(1):29-35. PMID: 38719683
Recombinase polymerase amplification (RPA) is an isothermal DNA amplification reaction at around 41 °C using recombinase (Rec), single-stranded DNA-binding protein (SSB), strand-displacing DNA polymerase (Pol), and an ATP-regenerating enzyme. Considering...
9.
Ikeda Y, Nishimoto S, Qiao Y, Yano H, Minami H, Ito M, et al.
J Pharmacol Toxicol Methods . 2024 Apr; 127:107508. PMID: 38670388
To measure α-glucosidase activity, rat intestinal acetone powder is commonly used as a source of α-glucosidase, and the mutarotase-glucose oxidase (GOD) methods commonly used to quantitate glucose produced by enzymatic...
10.
Juma K, Morimoto K, Sharma V, Sharma K, Biyani R, Biyani M, et al.
Mol Biol Rep . 2024 Apr; 51(1):574. PMID: 38664266
No abstract available.