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Shinsuke Fujiwara

Explore the profile of Shinsuke Fujiwara including associated specialties, affiliations and a list of published articles. Areas
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Recent Articles
1.
Odagaki Y, Murakami Y, Takita T, Mizutani K, Mikami B, Fujiwara S, et al.
Biochem Biophys Res Commun . 2024 Sep; 733:150728. PMID: 39321488
Agmatine, a natural polyamine also known as 4-aminobutyl-guanidine, is biosynthesized from arginine by decarboxylation. Aspergillus oryzae contains high amounts of agmatine, suggesting highly active arginine decarboxylase (ADC) in this organism....
2.
Hidese R, Ohira T, Sakakibara S, Suzuki T, Shigi N, Fujiwara S
mBio . 2024 Jul; 15(8):e0053424. PMID: 38975783
Ubiquitin-like proteins (Ubls) in eukaryotes and bacteria mediate sulfur transfer for the biosynthesis of sulfur-containing biomolecules and form conjugates with specific protein targets to regulate their functions. Here, we investigated...
3.
Wu H, Fujisawa Y, Tozuka Z, Fomenkov A, Nakura Y, Kajiyama S, et al.
Enzyme Microb Technol . 2024 Jul; 180:110471. PMID: 38959818
Here, we report a novel endonuclease and N-adenine DNA methyltransferase (mA methyltransferase) in the Ureaplasma parvum SV3F4 strain. Our previous study found that the SV3F4 strain carries 17 unique genes,...
4.
Ishii J, Nishikimi M, De Bus L, De Waele J, Takaba A, Kuriyama A, et al.
Microbiol Spectr . 2024 Jun; 12(7):e0034224. PMID: 38864641
Whether empirical therapy with carbapenems positively affects the outcomes of critically ill patients with bacterial infections remains unclear. This study aimed to investigate whether the use of carbapenems as the...
5.
Juma K, Murakami Y, Morimoto K, Takita T, Kojima K, Suzuki K, et al.
J Biosci Bioeng . 2024 May; 138(1):29-35. PMID: 38719683
Recombinase polymerase amplification (RPA) is an isothermal DNA amplification reaction at around 41 °C using recombinase (Rec), single-stranded DNA-binding protein (SSB), strand-displacing DNA polymerase (Pol), and an ATP-regenerating enzyme. Considering...
6.
Murakami Y, Ikuta S, Fukuda W, Akasaka N, Maruyama J, Shinma S, et al.
Appl Environ Microbiol . 2024 Apr; 90(5):e0029424. PMID: 38624200
spores, when sprinkled onto steamed rice and allowed to propagate, are referred to as rice ." Agmatine, a natural polyamine derived from arginine through the action of arginine decarboxylase (ADC),...
7.
Morimoto K, Juma K, Yamagata M, Takita T, Kojima K, Suzuki K, et al.
Mol Biol Rep . 2024 Feb; 51(1):367. PMID: 38411701
Background: Recombinase uvsY from bacteriophage T4, along with uvsX, is a key enzyme for recombinase polymerase amplification (RPA), which is used to amplify a target DNA sequence at a constant...
8.
Tanakura Y, Uekawa Y, Shige Y, Fukuda W, Ikuta S, Wu H, et al.
J Biotechnol . 2023 Dec; 380:38-50. PMID: 38135188
We evaluated the suitability of Komagataeibacter europaeus, a vinegar production organism adept at synthetic media growth, as a host for heterologous gene expression. Cryptic plasmids (pGE1 and pGE2 derivatives) from...
9.
Kojima K, Morimoto K, Juma K, Takita T, Saito K, Yanagihara I, et al.
J Biosci Bioeng . 2023 Sep; 136(5):341-346. PMID: 37718149
Recombinase polymerase amplification (RPA) is an isothermal DNA amplification reaction at around 41°C using recombinase (Rec), single-stranded DNA-binding protein (SSB), strand-displacing DNA polymerase (Pol), and an ATP-regenerating enzyme. In this...
10.
Juma K, Inoue E, Asada K, Fukuda W, Morimoto K, Yamagata M, et al.
J Biosci Bioeng . 2023 Feb; 135(4):282-290. PMID: 36806411
Recombinase polymerase amplification (RPA) is an isothermal DNA amplification reaction at around 41 °C using recombinase (Rec), single-stranded DNA-binding protein (SSB), and strand-displacing DNA polymerase (Pol). Component instability and the...