King C Chan
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Explore the profile of King C Chan including associated specialties, affiliations and a list of published articles.
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47
Citations
877
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Recent Articles
11.
Ye X, Chan K, Prieto D, Luke B, Johann Jr D, Stockwin L, et al.
Methods Mol Biol
. 2013 Apr;
1002:133-49.
PMID: 23625401
Differential (18)O/(16)O stable isotopic labeling that relies on post-digestion (18)O exchange is a simple and efficient method for the relative quantitation of proteins in complex mixtures. This method incorporates two...
12.
Ye X, Luke B, Johann Jr D, Chan K, Prieto D, Ono A, et al.
Methods Mol Biol
. 2012 Jun;
893:223-40.
PMID: 22665304
The role of membrane proteins is critical for regulation of physiologic and pathologic cellular processes. Hence it is not surpassing that membrane proteins make ∼70% of contemporary drug targets. Quantitative...
13.
Alexandre B, Charro N, Blonder J, Lopes C, Azevedo P, de Almeida A, et al.
J Proteomics
. 2012 Apr;
76 Spec No.:259-69.
PMID: 22538302
Structural and metabolic alterations in erythrocytes play an important role in the pathophysiology of Chronic Obstructive Pulmonary Disease (COPD). Whether these dysfunctions are related to the modulation of erythrocyte membrane...
14.
Issaq H, Fox S, Chan K, Veenstra T
J Sep Sci
. 2011 Nov;
34(24):3484-92.
PMID: 22102289
Chromatography and electrophoresis have been used for the last half-century to separate small and large molecules. Advances in MS instrumentation and techniques for sample introduction into the mass analyzer (i.e....
15.
Simoes T, Charro N, Blonder J, Faria D, Couto F, Chan K, et al.
J Proteomics
. 2011 May;
75(1):56-69.
PMID: 21621024
A comprehensive proteomic profiling of nasal epithelium (NE) is described. This study relies on simple subcellular fractionation used to obtain soluble- and membrane-enriched fractions followed by 2-dimensional liquid chromatography (2D-LC)...
16.
Chan K, Veenstra T, Issaq H
Anal Chem
. 2011 Feb;
83(6):2394-6.
PMID: 21319742
Proteomics is the study of all proteins in a biological sample. High-pressure liquid chromatography coupled online with mass spectrometry (HPLC/MS) is currently the method of choice for proteomic analysis. Proteins...
17.
Chan K, Veenstra T, Issaq H
Methods Mol Biol
. 2010 Dec;
697:145-53.
PMID: 21116963
Sensitive and fast analytical techniques are needed to determine the concentration of nanoparticles in biological samples (e.g., blood and tissues) for biodistribution and toxicity studies. This chapter describes a method...
18.
Das S, Bosley A, Ye X, Chan K, Chu I, Green J, et al.
J Proteome Res
. 2010 Oct;
9(12):6696-704.
PMID: 20968308
Affinity purification of protein complexes followed by identification using liquid chromatography/mass spectrometry (LC-MS/MS) is a robust method to study the fundamental process of protein interaction. Although affinity isolation reduces the...
19.
Ye X, Luke B, Johann Jr D, Ono A, Prieto D, Chan K, et al.
Anal Chem
. 2010 Jun;
82(13):5878-86.
PMID: 20540505
Differential (18)O/(16)O stable isotope labeling of peptides that relies on enzyme-catalyzed oxygen exchange at their carboxyl termini in the presence of H(2)(18)O has been widely used for relative quantitation of...
20.
de Ravin S, Zarember K, Long-Priel D, Chan K, Fox S, Gallin J, et al.
Blood
. 2010 Jun;
116(10):1755-60.
PMID: 20511543
In chronic granulomatous disease (CGD), defective phagocytic nicotinamide adenine dinucleotide phosphate (NADPH) oxidase activity causes reduced superoxide anion (O(2)(·)) radical production leading to frequent infections as well as granulomas and...