Isak S Pretorius
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Explore the profile of Isak S Pretorius including associated specialties, affiliations and a list of published articles.
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98
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3109
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Recent Articles
1.
Construction and iterative redesign of synXVI a 903 kb synthetic Saccharomyces cerevisiae chromosome
Goold H, Kroukamp H, Erpf P, Zhao Y, Kelso P, Calame J, et al.
Nat Commun
. 2025 Jan;
16(1):841.
PMID: 39833175
The Sc2.0 global consortium to design and construct a synthetic genome based on the Saccharomyces cerevisiae genome commenced in 2006, comprising 16 synthetic chromosomes and a new-to-nature tRNA neochromosome. In...
2.
Billerbeck S, Walker R, Pretorius I
Trends Biotechnol
. 2024 Apr;
42(9):1081-1096.
PMID: 38575438
Killer yeasts secrete protein toxins that are selectively lethal to other yeast and filamentous fungi. These exhibit exceptional genetic and functional diversity, and have several biotechnological applications. However, despite decades...
3.
Symons J, Dixon T, Dalziell J, Curach N, Paulsen I, Wiskich A, et al.
Nat Commun
. 2024 Mar;
15(1):2669.
PMID: 38531884
Engineering biology (EngBio) is a dynamic field that uses gene editing, synthesis, assembly, and engineering to design new or modified biological systems. EngBio applications could make a significant contribution to...
4.
Williams T, Kroukamp H, Xu X, Wightman E, Llorente B, Borneman A, et al.
Cell Genom
. 2023 Nov;
3(11):100379.
PMID: 38020977
Synthetic chromosome engineering is a complex process due to the need to identify and repair growth defects and deal with combinatorial gene essentiality when rearranging chromosomes. To alleviate these issues,...
5.
Dai J, Yang H, Pretorius I, Cai Y, Shen C, Chang M, et al.
Cell Genom
. 2023 Nov;
3(11):100441.
PMID: 38020973
The Synthetic Yeast Genome Project (Sc2.0) is an international collaboration that aims to create and optimize synthetic versions of each chromosome, with the ultimate goal of assembling a yeast organism...
6.
Dixon T, Walker R, Pretorius I
Yeast
. 2023 Sep;
40(10):443-456.
PMID: 37653687
Yeast research is entering into a new period of scholarship, with new scientific tools, new questions to ask and new issues to consider. The politics of emerging and critical technology...
7.
Badura J, van Wyk N, Zimmer K, Pretorius I, von Wallbrunn C, Wendland J
FEMS Yeast Res
. 2023 Jul;
23.
PMID: 37500280
Lack of gene-function analyses tools limits studying the biology of Hanseniaspora uvarum, one of the most abundant yeasts on grapes and in must. We investigated a rapid PCR-based gene targeting...
8.
van Wyk N, Binder J, Ludszuweit M, Kohler S, Brezina S, Semmler H, et al.
Foods
. 2023 May;
12(10).
PMID: 37238757
Traditional kombucha is a functional tea-based drink that has gained attention as a low or non-alcoholic beverage. The fermentation is conducted by a community of different microorganisms, collectively called SCOBY...
9.
Walker R, Pretorius I
Nat Food
. 2023 Apr;
3(4):249-254.
PMID: 37118192
Wine fermentation is a representation of complex higher-order microbial interactions. Despite the beneficial properties that these communities bring to wine, their complexity poses challenges in predicting the nature and outcome...
10.
Xu X, Meier F, Blount B, Pretorius I, Ellis T, Paulsen I, et al.
Nat Commun
. 2023 Apr;
14(1):1984.
PMID: 37031253
Naturally evolved organisms typically have large genomes that enable their survival and growth under various conditions. However, the complexity of genomes often precludes our complete understanding of them, and limits...