Iain M Hagan
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Explore the profile of Iain M Hagan including associated specialties, affiliations and a list of published articles.
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46
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1045
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Recent Articles
11.
Hagan I, Grallert A, Simanis V
Cold Spring Harb Protoc
. 2016 Aug;
2016(8).
PMID: 27480719
Here, we describe how the rapid reversibility of the nda3-KM311 cold-sensitive β-tubulin mutation was optimized by Mitsuhiro Yanagida's laboratory to synchronize mitotic progression in an entire cell population. The inability...
12.
Hagan I, Bagley S
Cold Spring Harb Protoc
. 2016 Jul;
2016(7).
PMID: 27371603
The acknowledged genetic malleability of fission yeast has been matched by impressive cytology to drive major advances in our understanding of basic molecular cell biological processes. In many of the...
13.
Hagan I
Cold Spring Harb Protoc
. 2016 Jul;
2016(7).
PMID: 27371599
Establishing the subcellular distribution of molecules of interest and the dynamics of their spatial control underpins all areas of cell and developmental biology. Although the ability to monitor the distribution...
14.
Hagan I, Grallert A, Simanis V
Cold Spring Harb Protoc
. 2016 Jun;
2016(6).
PMID: 27250945
Size selection of small cells from an asynchronous Schizosaccharomyces pombe culture offers a simple way to generate cultures in which progression through the mitotic cell division cycle is synchronized throughout...
15.
Hagan I, Grallert A, Simanis V
Cold Spring Harb Protoc
. 2016 Jun;
2016(6).
PMID: 27250944
Division of Schizosaccharomyces pombe by medial fission produces identically sized daughter cells that grow by tip extension until their own division is prompted by reaching the same critical size for...
16.
Hagan I
Cold Spring Harb Protoc
. 2016 Jun;
2016(6).
PMID: 27250943
The Schizosaccharomyces pombe filamentous (F)-actin cytoskeleton drives cell growth, morphogenesis, endocytosis, and cytokinesis. The protocol described here reveals the distribution of F-actin in fixed cells through the use of fluorescently...
17.
Hagan I
Cold Spring Harb Protoc
. 2016 Jun;
2016(6).
PMID: 27250942
Fission yeasts grow by tip extension, maintaining a constant width until they reach a critical size threshold and divide. Division by medial fission-which gives these yeast their name-generates a new...
18.
Fu J, Hagan I, Glover D
Cold Spring Harb Perspect Biol
. 2015 Feb;
7(2):a015800.
PMID: 25646378
The centrosome was discovered in the late 19th century when mitosis was first described. Long recognized as a key organelle of the spindle pole, its core component, the centriole, was...
19.
Grallert A, Boke E, Hagting A, Hodgson B, Connolly Y, Griffiths J, et al.
Nature
. 2014 Dec;
517(7532):94-98.
PMID: 25487150
The widespread reorganization of cellular architecture in mitosis is achieved through extensive protein phosphorylation, driven by the coordinated activation of a mitotic kinase network and repression of counteracting phosphatases. Phosphatase...
20.
Carpy A, Patel A, Tay Y, Hagan I, Macek B
Mol Cell Proteomics
. 2014 Nov;
14(1):243-50.
PMID: 25368411
Stable Isotope Labeling by Amino Acids (SILAC) is a commonly used method in quantitative proteomics. Because of compatibility with trypsin digestion, arginine and lysine are the most widely used amino...