Gregory Dame
Overview
Explore the profile of Gregory Dame including associated specialties, affiliations and a list of published articles.
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Snapshot
Articles
15
Citations
135
Followers
0
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Recent Articles
1.
Detection of Carbapenem Resistance Genes by qPCR: Choosing the Right Method for Total DNA Extraction
Heller C, Bachmann I, Spiegel M, Hufert F, Dame G
Microorganisms
. 2024 Jul;
12(7).
PMID: 39065054
Rapid and accurate detection of carbapenem resistance is important for infection control and targeted antibiotic therapy. PCR-based assay performance heavily depends on the quality and quantity of template DNA. Challenges...
2.
Bachmann I, Behrmann O, Klingenberg-Ernst M, Rupnik M, Hufert F, Dame G, et al.
Anal Chem
. 2024 Feb;
96(8):3267-3275.
PMID: 38358754
Nosocomial-associated diarrhea due to infection (CDI) is diagnosed after sample precultivation by the detection of the toxins in enzyme immunoassays or via toxin gene nucleic acid amplification. Rapid and direct...
3.
Dorendorf A, Bachmann I, Spiegel M, Abd El Wahed A, Dame G, Hufert F
J Clin Virol Plus
. 2022 Oct;
2(4):100115.
PMID: 36248766
No abstract available.
4.
Behrmann O, Bachmann I, Hufert F, Dame G
Biospektrum (Heidelb)
. 2020 Oct;
26(6):624-627.
PMID: 33078045
The COVID-19 pandemic highlights the need for fast and simple assays for nucleic acid detection. As an isothermal alternative to RT-qPCR, we outline the development of a detection scheme for...
5.
Behrmann O, Hugle M, Eckardt F, Bachmann I, Heller C, Schramm M, et al.
Micromachines (Basel)
. 2020 Jun;
11(6).
PMID: 32560308
We investigate the compatibility of three 3D printing materials towards real-time recombinase polymerase amplification (rtRPA). Both the general ability of the rtRPA reaction to occur while in contact with the...
6.
Behrmann O, Bachmann I, Spiegel M, Schramm M, Abd El Wahed A, Dobler G, et al.
Clin Chem
. 2020 May;
66(8):1047-1054.
PMID: 32384153
Background: The current outbreak of SARS-CoV-2 has spread to almost every country with more than 5 million confirmed cases and over 300,000 deaths as of May 26, 2020. Rapid first-line...
7.
Hugle M, Behrmann O, Raum M, Hufert F, Urban G, Dame G
Analyst
. 2020 Feb;
145(7):2554-2561.
PMID: 32072995
Nucleic acid amplification techniques such as real-time PCR are essential instruments for the identification and quantification of viruses. They are fast, very sensitive and highly specific, but often require elaborate...
8.
Behrmann O, Hugle M, Bronsert P, Herde B, Heni J, Schramm M, et al.
PLoS One
. 2019 Dec;
14(12):e0226571.
PMID: 31856234
We present a simple to operate microfluidic chip system that allows for the extraction of miRNAs from cells with minimal hands-on time. The chip integrates thermoelectric lysis (TEL) of cells...
9.
Dinter F, Burdukiewicz M, Schierack P, Lehmann W, Nestler J, Dame G, et al.
Anal Bioanal Chem
. 2019 Nov;
411(29):7725-7735.
PMID: 31760445
The rapid and simultaneous detection of DNA and protein biomarkers is necessary to detect the outbreak of a disease or to monitor a disease. For example, cardiovascular diseases are a...
10.
Cai D, Behrmann O, Hufert F, Dame G, Urban G
Sci Rep
. 2018 Feb;
8(1):3410.
PMID: 29467420
PCR inhibitors in clinical specimens negatively affect the sensitivity of diagnostic PCR and RT-PCR or may even cause false-negative results. To overcome PCR inhibition, increase the sensitivity of the assays...