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The COVID-19 pandemic highlights the need for fast and simple assays for nucleic acid detection. As an isothermal alternative to RT-qPCR, we outline the development of a detection scheme for SARS-CoV-2 RNA based on amplification (RT-RPA) technology. RPA uses recombination proteins in combination with a DNA polymerase for rapid amplification of target DNA at a constant temperature (39-42 °C) within 10 to 20 minutes and can be monitored in real-time with fluorescent probes.
Dual Gene Detection of H5N1 Avian Influenza Virus Based on Dual RT-RPA.
Wang Q, Wu S, Shuai J, Li Y, Fu X, Zhang M Molecules. 2024; 29(12).
PMID: 38930866 PMC: 11206350. DOI: 10.3390/molecules29122801.