Gregory D Gillispie
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Explore the profile of Gregory D Gillispie including associated specialties, affiliations and a list of published articles.
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12
Citations
309
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Recent Articles
1.
Schaaf T, Li A, Grant B, Peterson K, Yuen S, Bawaskar P, et al.
Biosensors (Basel)
. 2018 Oct;
8(4).
PMID: 30352972
We have developed fluorescence resonance energy transfer (FRET) biosensors with red-shifted fluorescent proteins (FP), yielding improved characteristics for time-resolved (lifetime) fluorescence measurements. In comparison to biosensors with green and red...
2.
Schaaf T, Peterson K, Grant B, Bawaskar P, Yuen S, Li J, et al.
SLAS Discov
. 2016 Dec;
22(3):262-273.
PMID: 27899691
A robust high-throughput screening (HTS) strategy has been developed to discover small-molecule effectors targeting the sarco/endoplasmic reticulum calcium ATPase (SERCA), based on a fluorescence microplate reader that records both the...
3.
Schaaf T, Peterson K, Grant B, Thomas D, Gillispie G
SLAS Discov
. 2016 Nov;
22(3):250-261.
PMID: 27879398
We have developed a microplate reader that records a complete high-quality fluorescence emission spectrum on a well-by-well basis under true high-throughput screening (HTS) conditions. The read time for an entire...
4.
Rebbeck R, Essawy M, Nitu F, Grant B, Gillispie G, Thomas D, et al.
SLAS Discov
. 2016 Oct;
22(2):176-186.
PMID: 27760856
Using time-resolved fluorescence resonance energy transfer (FRET), we have developed and validated the first high-throughput screening (HTS) method to discover compounds that modulate an intracellular Ca channel, the ryanodine receptor...
5.
Petersen K, Peterson K, Muretta J, Higgins S, Gillispie G, Thomas D
Rev Sci Instrum
. 2014 Nov;
85(11):113101.
PMID: 25430092
We describe a nanosecond time-resolved fluorescence spectrometer that acquires fluorescence decay waveforms from each well of a 384-well microplate in 3 min with signal-to-noise exceeding 400 using direct waveform recording....
6.
Gruber S, Cornea R, Li J, Peterson K, Schaaf T, Gillispie G, et al.
J Biomol Screen
. 2014 Jan;
19(2):215-22.
PMID: 24436077
We have used a "two-color" SERCA (sarco/endoplasmic reticulum calcium ATPase) biosensor and a unique high-throughput fluorescence lifetime plate reader (FLT-PR) to develop a high-precision live-cell assay designed to screen for...
7.
Cornea R, Gruber S, Lockamy E, Muretta J, Jin D, Chen J, et al.
J Biomol Screen
. 2012 Aug;
18(1):97-107.
PMID: 22923787
Using fluorescence resonance energy transfer (FRET), we performed a high-throughput screen (HTS) in a reconstituted membrane system, seeking compounds that reverse inhibition of sarcoplasmic reticulum Ca-ATPase (SERCA) by its cardiac...
8.
Gauer J, Sisk R, Murphy J, Jacobson H, Sutton R, Gillispie G, et al.
Biophys J
. 2012 Aug;
103(2):238-46.
PMID: 22853901
The C2A domain is one of two calcium ion (Ca(2+))- and membrane-binding domains within synaptotagmin I (Syt I), the identified Ca(2+) sensor for regulated exocytosis of neurotransmitter. We propose that...
9.
Lloyd W, Wilson R, Chang C, Gillispie G, Mycek M
Biomed Opt Express
. 2011 Jan;
1(2):574-586.
PMID: 21258491
A fiber-optic system was developed to rapidly acquire tissue fluorescence wavelength-time matrices (WTMs) with high signal-to-noise ratio (SNR). The essential system components (473 nm microchip laser operating at 3 kHz...
10.
Muretta J, Kyrychenko A, Ladokhin A, Kast D, Gillispie G, Thomas D
Rev Sci Instrum
. 2010 Nov;
81(10):103101.
PMID: 21034069
We describe a high-performance time-resolved fluorescence (HPTRF) spectrometer that dramatically increases the rate at which precise and accurate subnanosecond-resolved fluorescence emission waveforms can be acquired in response to pulsed excitation....