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Francisco Batista-Viera

Explore the profile of Francisco Batista-Viera including associated specialties, affiliations and a list of published articles. Areas
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Articles 16
Citations 104
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Recent Articles
11.
Puhl A, Giacomini C, Irazoqui G, Batista-Viera F, Villarino A, Terenzi H
Biotechnol Appl Biochem . 2008 Oct; 53(Pt 3):165-74. PMID: 18937642
Addition of tags [such as His (histidine) tags] is extremely helpful for the affinity purification of recombinant proteins. In several cases, these tags must be removed before performing functional and...
12.
Gonzalez-Pombo P, Perez G, Carrau F, Guisan J, Batista-Viera F, Brena B
Biotechnol Lett . 2008 Apr; 30(8):1469-75. PMID: 18414804
A collection of 60 non-Saccharomyces yeasts isolated from grape musts in Uruguayan vineyards was screened for beta-glucosidase activity and Metschnikowia pulcherrima was the best source of this enzyme activity. Its...
13.
Franco Fraguas L, Batista-Viera F, Carlsson J
J Chromatogr B Analyt Technol Biomed Life Sci . 2004 Apr; 803(2):237-41. PMID: 15063331
Preparation of Concanavalin A-adsorbents by immobilization on Sepharose activated with 1-cyano-4-(dimethylamino)-pyridinium tetrafluoroborate (CDAP-reagent) is reported. High immobilization yields of lectin (above 90%) were attained using an optimized CDAP-activating protocol. The...
14.
Grazu V, Ovsejevi K, Cuadra K, Betancor L, Manta C, Batista-Viera F
Appl Biochem Biotechnol . 2003 Aug; 110(1):23-32. PMID: 12909729
Disulfide reduction of Kluyveromyces lactis and Aspergillus oryzae beta-galactosidases and beta-lactoglobulin was assessed. Reduction was performed using one of two thiol-containing agents: dithiothreitol (DTT) or thiopropyl-agarose with a high degree...
15.
Franco-Fraguas L, Pla A, Ferreira F, Massaldi H, Suarez N, Batista-Viera F
J Chromatogr B Analyt Technol Biomed Life Sci . 2003 May; 790(1-2):365-72. PMID: 12767345
Optimized procedures for the affinity purification of soybean agglutinin (SBA) from soybean flour, and its further immobilization, were developed. Lectin purification on galactosyl-Sepharose yielded 44.5+/-3.5 mg of pure SBA/50 g...
16.
Irazoqui G, Villarino A, Batista-Viera F, Brena B
Biotechnol Bioeng . 2002 Jan; 77(4):430-4. PMID: 11787015
beta-Galactosidase (Escherichia coli) was immobilized through its thiol groups on thiolsulfinate-agarose gel. After enzyme immobilization, different nano-environments were generated by reacting the excess of gel-bound thiolsulfinate moieties with 2-mercaptoethanesulfonic acid...