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Erik Hoyt

Explore the profile of Erik Hoyt including associated specialties, affiliations and a list of published articles. Areas
Snapshot
Articles 7
Citations 67
Followers 0
Related Specialties
Chemistry
Biochemistry
Molecular Biology
Top 10 Co-Authors
Huijuan Li
Joseph P Smith
Zhijian Liu
Xiaodong Bu
Fengqiang Wang
Heather Lynaugh
Stephen R Hamilton
Sujatha Gomathinayagam
Erik Guetschow
Narayan Variankaval
Published In
Glycobiology
J Chromatogr A
Biotechnol Bioeng
Methods Mol Biol
J Pharm Biomed Anal
Affiliations
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Recent Articles
1.
Holistic analytical characterization and risk assessment of residual host cell protein impurities in an active pharmaceutical ingredient synthesized by biocatalysts
Wang F, Li X, Swanson M, Guetschow E, Winston M, Smith J, et al.
Biotechnol Bioeng . 2022 Apr; 119(8):2088-2104. PMID: 35437754
Host cell proteins (HCPs) are a significant class of process-related impurities commonly associated with the manufacturing of biopharmaceuticals. However, due to the increased use of crude enzymes as biocatalysts for...
2.
A kinase-cGAS cascade to synthesize a therapeutic STING activator
McIntosh J, Liu Z, Andresen B, Marzijarani N, Moore J, Marshall N, et al.
Nature . 2022 Mar; 603(7901):439-444. PMID: 35296845
The introduction of molecular complexity in an atom- and step-efficient manner remains an outstanding goal in modern synthetic chemistry. Artificial biosynthetic pathways are uniquely able to address this challenge by...
3.
Quantitation and speciation of residual protein within active pharmaceutical ingredients using image analysis with SDS-PAGE
Smith J, Ralbovsky N, Lauro M, Hoyt E, Guetschow E, Wang F, et al.
J Pharm Biomed Anal . 2021 Oct; 207:114393. PMID: 34607166
Recent advances in biocatalysis and directed enzyme evolution has led to a variety of enzymatically-driven, elegant processes for active pharmaceutical ingredient (API) production. For biocatalytic processes, quantitation of any residual...
4.
A cost-effective plate-based sample preparation for antibody N-glycan analysis
Burnina I, Hoyt E, Lynaugh H, Li H, Gong B
J Chromatogr A . 2013 Aug; 1307:201-6. PMID: 23932029
During early cell line and process development of therapeutic antibodies, a cost-effective high-throughput approach to characterize the N-linked glycans is highly desired given that a large number of samples need...
5.
N-glycosylamine-mediated isotope labeling for mass spectrometry-based quantitative analysis of N-linked glycans
Gong B, Hoyt E, Lynaugh H, Burnina I, Moore R, Thompson A, et al.
Anal Bioanal Chem . 2013 May; 405(17):5825-31. PMID: 23670280
N-linked glycosylation is a major protein modification involved in many essential cellular functions. Methods capable of quantitative glycan analysis are highly valuable and have been actively pursued. Here we describe...
6.
High-throughput multimodal strong anion exchange purification and N-glycan characterization of endogenous glycoprotein expressed in glycoengineered Pichia pastoris
Gomathinayagam S, Hoyt E, Thompson A, Brown E, Karaveg K, Hamilton S, et al.
Methods Mol Biol . 2012 Jun; 899:315-23. PMID: 22735962
The secretory pathway of the yeast Pichia pastoris has been engineered to produce complex human-type N-glycans (Choi et al., Proc Natl Acad Sci USA 100:5022-5027, 2003; Hamilton et al., Science...
7.
Elimination of β-mannose glycan structures in Pichia pastoris
Hopkins D, Gomathinayagam S, Rittenhour A, Du M, Hoyt E, Karaveg K, et al.
Glycobiology . 2011 Aug; 21(12):1616-26. PMID: 21840970
The methylotrophic yeast, Pichia pastoris, is an important organism used for the production of therapeutic proteins. However, the presence of fungal-like glycans, such as those containing β-mannose (Man) linkages, can...