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Dmitry A Tsybulsky

Explore the profile of Dmitry A Tsybulsky including associated specialties, affiliations and a list of published articles. Areas
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Recent Articles
1.
Povedailo V, Lysenko I, Tikhomirov S, Yakovlev D, Tsybulsky D, Kruhlik A, et al.
J Fluoresc . 2020 Apr; 30(3):629-635. PMID: 32300977
Bright fluorescent probes with enhanced intensities in the fluorescein channel are of great value for plenty of biological applications. To design effective probes one should introduce as many as possible...
2.
Tsybulsky D, Kvach M, Ryazantsev D, Aparin I, Stakheev A, Prokhorenko I, et al.
Mol Cell Probes . 2016 Oct; 30(5):285-290. PMID: 27720907
Molecular beacons carrying JOE dye (4',5'-dichloro-2',7'-dimethoxy-6-carboxyfluorescein) on a rigid or flexible linker and one or two BHQ1 quenchers have been prepared and tested in real-time PCR using Fusarium avenaceum elongation...
3.
Ryazantsev D, Kvach M, Tsybulsky D, Prokhorenko I, Stepanova I, Martynenko Y, et al.
Analyst . 2014 Apr; 139(11):2867-72. PMID: 24736939
Convenient preparation of fluorogenic hairpin DNA probes (molecular beacons) carrying a pair of FAM fluorophores (located close to 5'-terminus of the probe) or a pair of BHQ1 quenchers on 3'-terminus...
4.
Kvach M, Tsybulsky D, Shmanai V, Prokhorenko I, Stepanova I, Korshun V
Curr Protoc Nucleic Acid Chem . 2013 Mar; Chapter 4:4.55.1-4.55.33. PMID: 23512693
This unit describes the preparation of 5- and 6-carboxy derivatives of the xanthene fluorescent dyes fluorescein (FAM), 4',5'-dichloro-2',7'-dimethoxy-fluorescein (JOE), and tetramethylrhodamine (TAMRA) as individual isomers, and their conversion to non-nucleoside...
5.
Ryazantsev D, Tsybulsky D, Prokhorenko I, Kvach M, Martynenko Y, Philipchenko P, et al.
Anal Bioanal Chem . 2012 Jun; 404(1):59-68. PMID: 22710565
A typical TaqMan™ real-time PCR probe contains a 5'-fluorescent dye and a 3'-quencher. In the course of the amplification, the probe is degraded starting from the 5'-end, thus releasing fluorescent...
6.
Tsybulsky D, Kvach M, Stepanova I, Korshun V, Shmanai V
J Org Chem . 2011 Dec; 77(2):977-84. PMID: 22148236
A convenient procedure for the preparation of the fluorescent dye 4',5'-dichloro-2',7'-dimethoxy-5(6)-carboxyfluorescein (JOE) is reported; the overall yield achieved starting from isovanillin is 10 times higher (40% vs 4%) compared to...
7.
Kvach M, Tsybulsky D, Ustinov A, Stepanova I, Bondarev S, Gontarev S, et al.
Bioconjug Chem . 2007 Aug; 18(5):1691-6. PMID: 17696491
Pentafluorophenyl esters of 5- and 6-carboxyfluorescein-3',6'-O-dipivalate can be easily separated in multigram quantities by column chromatography. The individual isomers were converted into stable phosphoramidites suitable for oligonucleotide synthesis. The use...