Christina Kross
Overview
Explore the profile of Christina Kross including associated specialties, affiliations and a list of published articles.
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Articles
6
Citations
48
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Recent Articles
1.
Lingg N, Kross C, Engele P, Ohlknecht C, Koppl C, Fischer A, et al.
N Biotechnol
. 2022 Aug;
71:37-46.
PMID: 35926774
Fusion protein technologies improve the expression and purification of recombinant proteins, but the removal of the tags involved requires specific proteases. The circularly permuted caspase-2 (cpCasp2) with its specific cleavage...
2.
Koppl C, Lingg N, Fischer A, Kross C, Loibl J, Buchinger W, et al.
Int J Mol Sci
. 2022 Jul;
23(14).
PMID: 35887026
Fusion protein technologies to facilitate soluble expression, detection, or subsequent affinity purification in are widely used but may also be associated with negative consequences. Although commonly employed solubility tags have...
3.
Kross C, Engele P, Sprenger B, Fischer A, Lingg N, Baier M, et al.
J Biol Chem
. 2021 Aug;
297(4):101095.
PMID: 34418435
Proteases serve as important tools in biotechnology and as valuable drugs or drug targets. Efficient protein engineering methods to study and modulate protease properties are thus of great interest for...
4.
Ohlknecht C, Katz S, Kross C, Sprenger B, Engele P, Schneider R, et al.
J Chem Inf Model
. 2021 Feb;
61(3):1193-1203.
PMID: 33570387
Rational-design methods have proven to be a valuable toolkit in the field of protein design. Numerical approaches such as free-energy calculations or QM/MM methods are fit to widen the understanding...
5.
Cserjan-Puschmann M, Lingg N, Engele P, Kross C, Loibl J, Fischer A, et al.
Biomolecules
. 2020 Dec;
10(12).
PMID: 33255244
Caspase-2 is the most specific protease of all caspases and therefore highly suitable as tag removal enzyme creating an authentic N-terminus of overexpressed tagged proteins of interest. The wild type...
6.
Ohlknecht C, Petrov D, Engele P, Kross C, Sprenger B, Fischer A, et al.
Proteins
. 2020 May;
88(10):1303-1318.
PMID: 32432825
The N-terminal cleavage of fusion tags to restore the native N-terminus of recombinant proteins is a challenging task and up to today, protocols need to be optimized for different proteins...