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C Kleanthous

Explore the profile of C Kleanthous including associated specialties, affiliations and a list of published articles. Areas
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Articles 63
Citations 1316
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Recent Articles
1.
White G, Ottignon L, Georgiou T, Kleanthous C, MOORE G, Thomson A, et al.
J Magn Reson . 2007 Jan; 185(2):191-203. PMID: 17218133
X- and W-band EPR spectra, at room and low temperatures, are reported for nitroxide spin labels attached to cysteine residues selectively introduced into two proteins, the DNase domain of colicin-E9...
2.
Pommer A, Cal S, Keeble A, Walker D, Evans S, Kuhlmann U, et al.
J Mol Biol . 2001 Dec; 314(4):735-49. PMID: 11733993
Colicin endonucleases and the H-N-H family of homing enzymes share a common active site structural motif that has similarities to the active sites of a variety of other nucleases such...
3.
Kleanthous C, Walker D
Trends Biochem Sci . 2001 Oct; 26(10):624-31. PMID: 11590016
Immunity proteins are high affinity inhibitors of colicins--SOS-induced toxins released by bacteria during times of stress. Recent work has shown that nuclease-specific immunity proteins are exosite inhibitors, binding adjacent to...
4.
Gorski S, Capaldi A, Kleanthous C, Radford S
J Mol Biol . 2001 Sep; 312(4):849-63. PMID: 11575937
The helical bacterial immunity proteins Im7 and Im9 have been shown to fold via kinetic mechanisms of differing complexity, despite having 60 % sequence identity. At pH 7.0 and 10...
5.
Paget M, Bae J, Hahn M, Li W, Kleanthous C, Roe J, et al.
Mol Microbiol . 2001 Mar; 39(4):1036-47. PMID: 11251822
In the Gram-positive bacterium, Streptomyces coelicolor A3(2), expression of the thioredoxin system is modulated by a sigma factor called sigmaR in response to changes in the cytoplasmic thiol-disulphide status, and...
6.
Ferguson N, Li W, Capaldi A, Kleanthous C, Radford S
J Mol Biol . 2001 Mar; 307(1):393-405. PMID: 11243827
To address the role of sequence in the folding of homologous proteins, the folding and unfolding kinetics of the all-helical bacterial immunity proteins Im2 and Im9 were characterised, together with...
7.
Capaldi A, Shastry M, Kleanthous C, Roder H, Radford S
Nat Struct Biol . 2001 Jan; 8(1):68-72. PMID: 11135674
Many proteins populate partially organized structures during folding. Since these intermediates often accumulate within the dead time (2-5 ms) of conventional stopped-flow and quench-flow devices, it has been difficult to...
8.
Carr S, Walker D, James R, Kleanthous C, Hemmings A
Acta Crystallogr D Biol Crystallogr . 2000 Nov; 56(Pt 12):1630-3. PMID: 11092930
The complex between the ribonuclease domain of the ribosome-inactivating colicin E3 and its protein inhibitor, the cognate immunity Im3, has been crystallized and preliminary X-ray characterization has been performed. Single...
9.
Penfold C, Hemmings A, MOORE G, Kleanthous C, James R
Mol Microbiol . 2000 Nov; 38(3):639-49. PMID: 11069686
The mechanism by which E colicins recognize and then bind to BtuB receptors in the outer membrane of Escherichia coli cells is a poorly understood first step in the process...
10.
Boetzel R, Czisch M, Kaptein R, Hemmings A, James R, Kleanthous C, et al.
Protein Sci . 2000 Oct; 9(9):1709-18. PMID: 11045617
The bacterial toxin colicin E9 is secreted by producing Escherichia coli cells with its 9.5 kDa inhibitor protein Im9 bound tightly to its 14.5 kDa C-terminal DNase domain. Double- and...