Expression of Epstein-barr Virus Nuclear Antigen 1 is Associated with Enhanced Expression of CD25 in the Hodgkin Cell Line L428

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1999 Jan 9

PMID 9882370

Citations 20

Authors

D Kube

M Vockerodt

O Weber

K Hell

J Wolf

B Haier

F A Grasser

N Muller-Lantzsch

E Kieff

V Diehl

H Tesch

Affiliations

Soon will be listed here.

Abstract

Epstein-Barr virus is associated with several human malignancies including Burkitt's lymphoma, nasopharyngeal carcinoma, and Hodgkin's disease (HD). To examine the effect of Epstein-Barr virus nuclear antigen 1 (EBNA-1) in the pathogenesis of HD, we transfected the gene into the HD cell line L428. EBNA-1 expression was associated with significantly enhanced CD25 expression (interleukin 2 [IL-2]-receptor alpha chain) in transient and stably transfected L428 cells but did not affect the expression of IL-2 receptor beta and gamma chains. There was no up-regulation of the B-cell activation molecules CD23, CD30, CD39, CD40, CD44, CD71, and CD54 (intercellular adhesion molecule 1) or enhanced production of IL-6, IL-10, lymphotoxin alpha, and the soluble form of CD25. Stable EBNA-1-expressing L428 cells were nontumorigenic in SCID mice but showed enhanced lymphoma development in nonobese diabetic-SCID mice compared to mock-transfected cells.

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References

Oudejans J, Dukers D, Jiwa N, van den Brule A, Grasser F, de Bruin P . Expression of epstein-barr virus encoded nuclear antigen 1 in benign and malignant tissues harbouring EBV. J Clin Pathol. 1996; 49(11):897-902. PMC: 500828. DOI: 10.1136/jcp.49.11.897. View

von Kalle C, Wolf J, Becker A, Sckaer A, Munck M, Engert A . Growth of Hodgkin cell lines in severely combined immunodeficient mice. Int J Cancer. 1992; 52(6):887-91. DOI: 10.1002/ijc.2910520610. View

Deacon E, Pallesen G, Niedobitek G, Crocker J, Brooks L, Rickinson A . Epstein-Barr virus and Hodgkin's disease: transcriptional analysis of virus latency in the malignant cells. J Exp Med. 1993; 177(2):339-49. PMC: 2190903. DOI: 10.1084/jem.177.2.339. View

Tesch H, Jucker M, Klein S, Abts H, Gunther A, Krueger G . Hodgkin and Reed-Sternberg cells express interleukin 6 and interleukin 6 receptors. Leuk Lymphoma. 1992; 7(4):297-303. DOI: 10.3109/10428199209049781. View

Klein S, Jucker M, Diehl V, Tesch H . Production of multiple cytokines by Hodgkin's disease derived cell lines. Hematol Oncol. 1992; 10(6):319-29. DOI: 10.1002/hon.2900100605. View

Tesch H, Gunther A, Abts H, Jucker M, Klein S, Krueger G . Expression of interleukin-2R alpha and interleukin-2R beta in Hodgkin's disease. Am J Pathol. 1993; 142(6):1714-20. PMC: 1886973. View

Burton J, Kay N . Does IL-2 receptor expression and secretion in chronic B-cell leukemia have a role in down-regulation of the immune system?. Leukemia. 1994; 8(1):92-6. View

Tsilivakos V, Tsapis A, Kakolyris S, Iliakis P, Perraki M, Georgoulias V . Characterization of interleukin 2 receptors on B-cell chronic lymphocytic leukemia cells. Leukemia. 1994; 8(9):1571-8. View

Grasser F, Murray P, Kremmer E, Klein K, Remberger K, Feiden W . Monoclonal antibodies directed against the Epstein-Barr virus-encoded nuclear antigen 1 (EBNA1): immunohistologic detection of EBNA1 in the malignant cells of Hodgkin's disease. Blood. 1994; 84(11):3792-8. View

10.

Snudden D, Hearing J, Smith P, Grasser F, Griffin B . EBNA-1, the major nuclear antigen of Epstein-Barr virus, resembles 'RGG' RNA binding proteins. EMBO J. 1994; 13(20):4840-7. PMC: 395423. DOI: 10.1002/j.1460-2075.1994.tb06810.x. View

11.

Sage D, Stein L, Groelle H, Fingeroth J . Expression of recombination activating genes (RAG-1 and RAG-2) in Epstein-Barr virus-bearing B cells. Blood. 1995; 85(5):1289-99. View

12.

Chen F, Zou J, Di Renzo L, Winberg G, Hu L, Klein E . A subpopulation of normal B cells latently infected with Epstein-Barr virus resembles Burkitt lymphoma cells in expressing EBNA-1 but not EBNA-2 or LMP1. J Virol. 1995; 69(6):3752-8. PMC: 189092. DOI: 10.1128/JVI.69.6.3752-3758.1995. View

13.

Totero D, Francia di Celle P, Cignetti A, Foa R . The IL-2 receptor complex: expression and function on normal and leukemic B cells. Leukemia. 1995; 9(9):1425-31. View

14.

Gorschluter M, Bohlen H, Hasenclever D, Diehl V, Tesch H . Serum cytokine levels correlate with clinical parameters in Hodgkin's disease. Ann Oncol. 1995; 6(5):477-82. DOI: 10.1093/oxfordjournals.annonc.a059218. View

15.

Willerford D, Chen J, Ferry J, Davidson L, Ma A, Alt F . Interleukin-2 receptor alpha chain regulates the size and content of the peripheral lymphoid compartment. Immunity. 1995; 3(4):521-30. DOI: 10.1016/1074-7613(95)90180-9. View

16.

Hsu M, Chen Y, Chang Y, Ko J, Sheen T . Soluble interleukin-2 receptor as a clinical parameter for nasopharyngeal carcinoma. Asian Pac J Allergy Immunol. 1995; 13(1):1-4. View

17.

Vasef M, Kamel O, Chen Y, Medeiros L, Weiss L . Detection of Epstein-Barr virus in multiple sites involved by Hodgkin's disease. Am J Pathol. 1995; 147(5):1408-15. PMC: 1869524. View

18.

Srinivas S, Sixbey J . Epstein-Barr virus induction of recombinase-activating genes RAG1 and RAG2. J Virol. 1995; 69(12):8155-8. PMC: 189773. DOI: 10.1128/JVI.69.12.8155-8158.1995. View

19.

Khan G, Naase M . Down-regulation of Epstein-Barr virus nuclear antigen 1 in Reed-Sternberg cells of Hodgkin's disease. J Clin Pathol. 1995; 48(9):845-8. PMC: 502874. DOI: 10.1136/jcp.48.9.845. View

20.

Herbst H, Foss H, Samol J, Araujo I, Klotzbach H, Krause H . Frequent expression of interleukin-10 by Epstein-Barr virus-harboring tumor cells of Hodgkin's disease. Blood. 1996; 87(7):2918-29. View