A Subpopulation of Normal B Cells Latently Infected with Epstein-Barr Virus Resembles Burkitt Lymphoma Cells in Expressing EBNA-1 but Not EBNA-2 or LMP1

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1995 Jun 1

PMID 7745723

Citations 68

Authors

F Chen

J Z Zou

L Di Renzo

G Winberg

L F Hu

E Klein

G Klein

I Ernberg

Affiliations

Soon will be listed here.

Abstract

Using reverse transcription of whole cellular RNA and nested PCR, we have performed experiments mixing different proportions of Epstein-Barr virus (EBV)-carrying and EBV-negative cells. Based on the results, a method that detects viral transcripts for EBNA-1, EBNA-2, LMP1, and LMP2a from less than one positive cell among 10(5) negative cells was developed. With this method we have shown that the EBV DNA positive cells among small, high-density peripheral blood B-lymphocytes of normal healthy persons express EBNA-1-mRNA but not EBNA-2 or LMP1. A similar EBV expression pattern is found in type I Burkitt lymphoma cells. We suggest that the expression pattern in the lymphoma cells reflects the viral strategy in normal resting B cells and meets the requirements of latent persistence.

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