Characterization of Reciprocal Translocations in Pigs Using Dual-colour Chromosome Painting and Primed in Situ DNA Labelling

Overview

Journal Chromosome Res

Date 1999 Jan 1

PMID 9872665

Citations 4

Authors

A Pinton

A Ducos

A Seguela

H M Berland

R Darre

A Darre

P Pinton

A Schmitz

E P Cribiu

M Yerle

Affiliations

Soon will be listed here.

Abstract

We report the use of dual-colour chromosome painting to determine the exact nature of certain chromosome rearrangements observed in the pig (Sus scrofa domestica). The chromosomal abnormalities were detected by GTG- and RBG-banding techniques. The initially proposed interpretations were: (1) rcp(6;13)(p1.5;q4.1); (2) rcp(11;16)(p1.4;q1.4); (3) rcp(6;16)(p1.1;q1.1); (4) rcp(13;17)(q4.1;q1.1); (5) rcp(6;14)(q2.7;q2.1); (6) rcp(3;5)(p1.3;q2.3); (7) rcp(2; 14)(q1.3;q2.7); (8) rcp(15;17)(q1.3;q2.1). Hybridizations were carried out with biotin- and digoxigenin-labelled probes obtained by priming authorizing random mismatches polymerase chain reaction (PARM-PCR) amplification of porcine flow-sorted chromosomes. In some cases, i.e. (1), (4), (5), (6), (7) and (8), the fluorescence in situ hybridization (FISH) results allowed confirmation of the interpretations proposed with classical cytogenetic methods. Chromosome painting proved the reciprocity of the translocation in cases (1), (6) and (8), whereas modifications of the formula were proposed for case (2). Primed in situ DNA labelling (PRINS) experiments have also been carried out in case (3) using a primer specific for the centromeres of acrocentric chromosomes (first experiment) or a primer specific for the centromeres of a subset of meta- and submetacentric chromosomes including chromosome 6 (second experiment). It allowed us to demonstrate that the breakpoints occurred in the centromeric region of chromosome 16 and in the p. arm of chromosome 6, just above the centromere.

Citing Articles

Classical, Molecular, and Genomic Cytogenetics of the Pig, a Clinical Perspective.

Donaldson B, Villagomez D, King W Animals (Basel). 2021; 11(5).

PMID: 33925534 PMC: 8146943. DOI: 10.3390/ani11051257.

A new case of reciprocal translocation t(10;13)(q16;q21) diagnosed in an AI boar.

Danielak-Czech B, Slota E J Appl Genet. 2007; 48(4):379-88.

PMID: 17998596 DOI: 10.1007/BF03195236.

Comparison of spontaneous background genomic aberration frequencies among cattle, pig and humans using dual-colored FISH.

Rezacova O, Kubickova S, Cernohorska H, Rubes J Chromosome Res. 2003; 11(7):715-24.

PMID: 14606633 DOI: 10.1023/a:1025941827523.

The use of laser microdissection for the preparation of chromosome-specific painting probes in farm animals.

Kubickova S, Cernohorska H, Musilova P, Rubes J Chromosome Res. 2002; 10(7):571-7.

PMID: 12498346 DOI: 10.1023/a:1020914702767.

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