The Use of Laser Microdissection for the Preparation of Chromosome-specific Painting Probes in Farm Animals

Overview

Journal Chromosome Res

Date 2002 Dec 25

PMID 12498346

Citations 55

Authors

Svatava Kubickova

Halina Cernohorska

Petra Musilova

Jiri Rubes

Affiliations

Soon will be listed here.

Abstract

Laser microbeam microdissection and laser pressure catapulting procedure were used for the construction of chromosome-specific painting probes, arm-specific probes and probes for chromosomal subfragments. We report on a method for generation of fluorescence in-situ hybridization probes from laser dissected chromosomes of farm animals. So far, using the described method, a set of chromosome-specific painting probes has been obtained for all porcine chromosomes, 17 chromosomes of cattle and selected equine chromosomes. It is concluded that the laser technology appears to be a useful and powerful tool for the construction of chromosome-specifi c painting probes. Its main advantage is the fast non-contact collection of chromosomes.

Citing Articles

From cytogenetics to cytogenomics: a new era in the diagnosis of chromosomal abnormalities in domestic animals.

Switonski M, Szczerbal I, Nowacka-Woszuk J J Appl Genet. 2025; .

PMID: 39869248 DOI: 10.1007/s13353-025-00943-x.

Zygosity-based sex determination in a butterfly drives hypervariability of .

Vant Hof A, Whiteford S, Yung C, Yoshido A, Zrzava M, de Jong M Sci Adv. 2024; 10(18):eadj6979.

PMID: 38701204 PMC: 11067997. DOI: 10.1126/sciadv.adj6979.

Comparative karyotype analysis of the red brocket deer (M. americana sensu lato and M. rufa) complex: evidence of drastic chromosomal evolution and implications on speciation process.

Bernegossi A, Galindo D, Peres P, Vozdova M, Cernohorska H, Kubickova S J Appl Genet. 2024; 65(3):601-614.

PMID: 38662189 DOI: 10.1007/s13353-024-00861-4.

X Chromosome-Specific Repeats in Non-Domestic Bovidae.

Kubickova S, Kopecna O, Cernohorska H, Rubes J, Vozdova M Genes (Basel). 2024; 15(2).

PMID: 38397149 PMC: 10887555. DOI: 10.3390/genes15020159.

Cytogenetic Analysis of the Fish Genus Carassius Indicates Divergence, Fission, and Segmental Duplication as Drivers of Tandem Repeat and Microchromosome Evolution.

Fornaini N, Cernohorska H, do Vale Martins L, Knytl M Genome Biol Evol. 2024; 16(3).

PMID: 38340334 PMC: 11079324. DOI: 10.1093/gbe/evae028.

References

Bohlander S, Espinosa 3rd R, Le Beau M, Rowley J, Diaz M . A method for the rapid sequence-independent amplification of microdissected chromosomal material. Genomics. 1992; 13(4):1322-4. DOI: 10.1016/0888-7543(92)90057-y. View

Lear T, Layton G . Use of zoo-FISH to characterise a reciprocal translocation in a thoroughbred mare: t(1;1 6)(q16;q21.3). Equine Vet J. 2002; 34(2):207-9. DOI: 10.2746/042516402776767295. View

Makinen A, Andersson M, Nikunen S . Detection of the X chromosomes in a Klinefelter boar using a whole human X chromosome painting probe. Anim Reprod Sci. 1998; 52(4):317-23. DOI: 10.1016/s0378-4320(98)00136-5. View

Muller-Navia J, Nebel A, SCHLEIERMACHER E . Complete and precise characterization of marker chromosomes by application of microdissection in prenatal diagnosis. Hum Genet. 1995; 96(6):661-7. DOI: 10.1007/BF00210295. View

Telenius H, Carter N, Bebb C, Nordenskjold M, Ponder B, Tunnacliffe A . Degenerate oligonucleotide-primed PCR: general amplification of target DNA by a single degenerate primer. Genomics. 1992; 13(3):718-25. DOI: 10.1016/0888-7543(92)90147-k. View

Iannuzzi L, Molteni L, Di Meo G, Perucatti A, Lorenzi L, Incarnato D . A new balanced autosomal reciprocal translocation in cattle revealed by banding techniques and human-painting probes. Cytogenet Cell Genet. 2002; 94(3-4):225-8. DOI: 10.1159/000048820. View

Goldammer T, Weikard R, Brunner R, Schwerin M . Generation of chromosome fragment specific bovine DNA sequences by microdissection and DOP-PCR. Mamm Genome. 1996; 7(4):291-6. DOI: 10.1007/s003359900085. View

Breen M, Langford C, Carter N, Fischer P, Marti E, Gerstenberg C . Detection of equine X chromosome abnormalities in equids using a horse X whole chromosome paint probe (WCPP). Vet J. 1997; 153(3):235-8. DOI: 10.1016/s1090-0233(97)80057-3. View

Raudsepp T, Chowdhary B . Construction of chromosome-specific paints for meta- and submetacentric autosomes and the sex chromosomes in the horse and their use to detect homologous chromosomal segments in the donkey. Chromosome Res. 1999; 7(2):103-14. DOI: 10.1023/a:1009234814635. View

10.

Chaudhary R, Kijas J, Raudsepp T, Guan X, Zhang H, Chowdhary B . Microdissection of pig chromosomes: dissection of whole chromosomes, arms and bands for construction of paints and libraries. Hereditas. 1998; 128(3):265-71. DOI: 10.1111/j.1601-5223.1998.00265.x. View

11.

Pinton A, Ducos A, Berland H, Seguela A, Brun-Baronnat C, Darre A . Chromosomal abnormalities in hypoprolific boars. Hereditas. 2000; 132(1):55-62. DOI: 10.1111/j.1601-5223.2000.00055.x. View

12.

Schermelleh L, Thalhammer S, Heckl W, Posl H, Cremer T, Schutze K . Laser microdissection and laser pressure catapulting for the generation of chromosome-specific paint probes. Biotechniques. 1999; 27(2):362-7. DOI: 10.2144/99272rr04. View

13.

Schmitz A, Chardon P, Gainche I, Chaput B, Guilly M, Frelat G . Pig standard bivariate flow karyotype and peak assignment for chromosomes X, Y, 3, and 7. Genomics. 1992; 14(2):357-62. DOI: 10.1016/s0888-7543(05)80226-5. View

14.

Guan X, Trent J, Meltzer P . Generation of band-specific painting probes from a single microdissected chromosome. Hum Mol Genet. 1993; 2(8):1117-21. DOI: 10.1093/hmg/2.8.1117. View

15.

Pinton A, Ducos A, Seguela A, Berland H, Darre R, Darre A . Characterization of reciprocal translocations in pigs using dual-colour chromosome painting and primed in situ DNA labelling. Chromosome Res. 1999; 6(5):361-6. DOI: 10.1023/a:1009244415357. View

16.

Weimer J, Kiechle M, Senger G, Wiedemann U, Ovens-Raeder A, Schuierer S . An easy and reliable procedure of microdissection technique for the analysis of chromosomal breakpoints and marker chromosomes. Chromosome Res. 1999; 7(5):355-62. DOI: 10.1023/a:1009263913478. View

17.

Christian A, Garcia H, Tucker J . PCR in situ followed by microdissection allows whole chromosome painting probes to be made from single microdissected chromosomes. Mamm Genome. 1999; 10(6):628-31. DOI: 10.1007/s003359901058. View

18.

Fend F, Raffeld M . Laser capture microdissection in pathology. J Clin Pathol. 2000; 53(9):666-72. PMC: 1731250. DOI: 10.1136/jcp.53.9.666. View

19.

Dixon S, Miller N, Carter N, Tucker E . Bivariate flow cytometry of farm animal chromosomes: a potential tool for gene mapping. Anim Genet. 1992; 23(3):203-10. DOI: 10.1111/j.1365-2052.1992.tb00132.x. View