Production of Beta-defensins by Human Airway Epithelia

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1998 Dec 9

PMID 9843998

Citations 183

Authors

P K Singh

H P Jia

K Wiles

J Hesselberth

L Liu

B A Conway

E P Greenberg

E V Valore

M J Welsh

T Ganz

B F Tack

P B McCray Jr

Affiliations

Soon will be listed here.

Abstract

Human beta-defensins (HBDs) are antimicrobial peptides that may play a role in mucosal defense. Diminished activity of these peptides has been implicated in the pathogenesis of cystic fibrosis (CF) lung disease. We show that HBD-1 and HBD-2 mRNAs are expressed in excised surface and submucosal gland epithelia from non-CF and CF patients. The pro-inflammatory cytokine interleukin-1beta stimulated the expression of HBD-2 but not HBD-1 mRNA and peptide in primary cultures of airway epithelia. HBD-1 was found in bronchoalveolar lavage (BAL) fluid from normal volunteers, CF patients, and patients with inflammatory lung diseases, whereas HBD-2 was detected in BAL fluid from patients with CF or inflammatory lung diseases, but not in normal volunteers. Both HBD-1 and HBD-2 were found in BAL fluid in concentrations of several ng/ml, and both recombinant peptides showed salt-sensitive bactericidal activity. These data suggest that in the lung HBD-2 expression is induced by inflammation, whereas HBD-1 may serve as a defense in the absence of inflammation.

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