Effective Inhibition of Influenza Virus Production in Cultured Cells by External Guide Sequences and Ribonuclease P

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1998 Jun 24

PMID 9636148

Citations 40

Authors

D Plehn-Dujowich

S Altman

Affiliations

Soon will be listed here.

Abstract

The polymerase (PB2) and nucleocapsid (NP) genes encoded by the genome of influenza virus are essential for replication of the virus. When synthetic genes that express RNAs for external guide sequences targeted to the mRNAs of the PB2 and NP genes are stably incorporated into mouse cells in tissue culture, infection of these cells with influenza virus is nonproductive. Endogenous RNase P cleaves the targeted influenza virus mRNAs when they are in a complex with the external guide sequences. Targeting two different mRNAs simultaneously inhibits viral particle production more efficiently than does targeting only one mRNA.

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